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Literature summary for 1.8.1.13 extracted from
- The function of gamma-glutamylcysteine and bis-gamma-glutamylcystine reductase in Halobacterium halobium (1989), J. Biol. Chem., 264, 719-725.
General Stability
| General Stability | Organism |
|---|---|
| low-ionic-strength buffer below 1.0 M leads to rapid denaturation | Halobacterium salinarum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AsO2- | 25 mM arsenite, relative activity 46% | Halobacterium salinarum |  |
| Cu2+ | relative activity less than 5% | Halobacterium salinarum | |
| Hg2+ | relative activity less than 5% | Halobacterium salinarum | |
| Zn2+ | relative activity less than 44% | Halobacterium salinarum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.29 | - |
NADPH | - |
Halobacterium salinarum | |
| 0.81 | - |
bis-gamma-glutamylcystine | - |
Halobacterium salinarum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Halobacterium salinarum | 5737 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 61000 | - |
2 * 61000, SDS-PAGE | Halobacterium salinarum |
| 122000 | - |
- |
Halobacterium salinarum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| bis-gamma-glutamylcystine + NADPH + H+ | Halobacterium salinarum | - |
2 gamma-glutamylcysteine + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Halobacterium salinarum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Halobacterium salinarum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 28 | - |
- |
Halobacterium salinarum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH | - |
Halobacterium salinarum | 4-nitrobenzenethiol + NADP+ | - |
? | |
| bis-gamma-glutamylcystine + NADPH + H+ | - |
Halobacterium salinarum | 2 gamma-glutamylcysteine + NADP+ | - |
? | |
| additional information | no activity detected with GSSG | Halobacterium salinarum | ? | - |
? | |
| additional information | lipoic acid disulfide and dihydrolipoamide are no substrates | Halobacterium salinarum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 61000, SDS-PAGE | Halobacterium salinarum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GCR | - |
Halobacterium salinarum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 28.3 | - |
FAD | - |
Halobacterium salinarum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Halobacterium salinarum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.3 | 8.2 | about half-maximal activity at pH 6.3 and 8.2 | Halobacterium salinarum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | flavoprotein, 1 mol FAD/mol subunit | Halobacterium salinarum | |
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