Application | Comment | Organism |
---|---|---|
brewing | mutations in the sulfite reductase genes, MET5 and MET10, are responsible for low H2S phenotypes, yeast strains with reduced H2S production offer promising solutions to H2S-related taints generated during wine production | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
the MET5 and Met10 coding sequences are cloned into the vector pGEM-T-easy and subsequently into the centromeric Saccharomyces cerevisiae expression plasmid pRS416 | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
A979T | Met5p mutant | Saccharomyces cerevisiae |
E1356K | Met5p mutant | Saccharomyces cerevisiae |
E929K | Met10p mutant | Saccharomyces cerevisiae |
G1115D | Met5p mutant | Saccharomyces cerevisiae |
L606F | Met10p mutant | Saccharomyces cerevisiae |
T990I | Met10p mutant | Saccharomyces cerevisiae |
T997I | Met10p mutant | Saccharomyces cerevisiae |
W59X | Met10p mutant | Saccharomyces cerevisiae |
W841X | Met10p mutant | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
hydrogen sulfide + NADP+ + H2O | Saccharomyces cerevisiae | - |
sulfite + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
allele 1, subunit beta, MET5, subunit alpha, MET10 | - |
Saccharomyces cerevisiae | - |
allele 2, subunit beta, MET5, subunit alpha, MET10 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hydrogen sulfide + NADP+ + H2O | - |
Saccharomyces cerevisiae | sulfite + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | two alpha- and two beta-subunits, encoded by the MET10 and MET5 gene, respectively | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
sulfite reductase | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Saccharomyces cerevisiae | |
FMN | - |
Saccharomyces cerevisiae | |
iron-sulfur centre | - |
Saccharomyces cerevisiae | |
NADPH | - |
Saccharomyces cerevisiae | |
siroheme | - |
Saccharomyces cerevisiae |