Inhibitors | Comment | Organism | Structure |
---|---|---|---|
arsenite | reversible inactivation of lipoamide-reducing reaction, no decrease in diaphorase activity | Mycolicibacterium smegmatis | |
NAD+ | substrate inhibition. The rate of lipoamide reduction is dependent upon the NAD+/NADH ratio, the reaction is activated at low ratios and inhibited at high ratios | Mycolicibacterium smegmatis | |
NADH | substrate inhibition. The rate of lipoamide reduction is dependent upon the NAD+/NADH ratio, the reaction is activated at low ratios and inhibited at high ratios | Mycolicibacterium smegmatis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
1,4-benzoquinone | - |
Mycolicibacterium smegmatis | |
0.05 | - |
1,4-Naphthoquinone | - |
Mycolicibacterium smegmatis | |
1.3 | - |
Lipoamide | - |
Mycolicibacterium smegmatis | |
2.9 | - |
lipoate | - |
Mycolicibacterium smegmatis | |
4.7 | - |
dihydrolipoate | - |
Mycolicibacterium smegmatis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49342 | - |
x * 49342, electrospray mass spectrometry | Mycolicibacterium smegmatis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium smegmatis | - |
- |
- |
Pisum sativum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Mycolicibacterium smegmatis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
protein N6-(dihydrolipoyl)lysine + NAD+ = protein N6-(lipoyl)lysine + NADH + H+ | ping-pong mechanism | Mycolicibacterium smegmatis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.43 | - |
- |
Mycolicibacterium smegmatis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,4-benzoquinone + NADH | - |
Mycolicibacterium smegmatis | 1,4-benzoquinol + NAD+ | - |
? | |
dihydrolipoamide + NAD+ | - |
Mycolicibacterium smegmatis | lipoamide + NADH | - |
? | |
dihydrolipoamide + NAD+ | - |
Pisum sativum | lipoamide + NADH | - |
? | |
dihydrolipoate + NAD+ | - |
Mycolicibacterium smegmatis | lipoate + NADH + H+ | - |
r | |
lipoate + NADH + H+ | - |
Mycolicibacterium smegmatis | dihydrolipoate + NAD+ | - |
r | |
naphthoquinone + NADH | - |
Mycolicibacterium smegmatis | 1,4-naphthoquinol + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | - |
Pisum sativum |
? | x * 49342, electrospray mass spectrometry | Mycolicibacterium smegmatis |
More | - |
Pisum sativum |
More | the enzyme oligomerizes to a high-molecular weight species, above 300000 Da, under nondenaturing conditions | Mycolicibacterium smegmatis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pisum sativum | |
NAD+ | the rate of lipoamide reduction is dependent upon the NAD+/NADH ratio, the reaction is activated at low ratios and inhibited at high ratios | Mycolicibacterium smegmatis | |
NADH | the rate of lipoamide reduction is dependent upon the NAD+/NADH ratio, the reaction is activated at low ratios and inhibited at high ratios | Mycolicibacterium smegmatis |