Cloned (Comment) | Organism |
---|---|
overexpressed in the parent organism by using the halophilic archaeal rRNA promoter | Haloferax volcanii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
dihydrolipoamide | pH 8.0, 37°C | Haloferax volcanii | |
0.08 | - |
NAD+ | pH 8.0, 37°C | Haloferax volcanii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | maximal enzymatic activity at 2 to 3 M KCl | Haloferax volcanii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66500 | - |
x * 66500, SDS-PAGE | Haloferax volcanii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haloferax volcanii | Q04829 | - |
- |
Haloferax volcanii DSM 3757 | Q04829 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Haloferax volcanii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
24 | - |
pH 8.0, 37°C | Haloferax volcanii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydrolipoamide + NAD+ | - |
Haloferax volcanii | lipoamide + NADH | - |
? | |
dihydrolipoamide + NAD+ | - |
Haloferax volcanii DSM 3757 | lipoamide + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 66500, SDS-PAGE | Haloferax volcanii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Haloferax volcanii |