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Literature summary for 1.8.1.5 extracted from

  • Kofoed, M.A.; Wampler, D.A.; Pandey, A.S.; Peters, J.W.; Ensign, S.A.
    Roles of the redox-active disulfide and histidine residues forming a catalytic dyad in reactions catalyzed by 2-ketopropyl coenzyme M oxidoreductase/carboxylase (2011), J. Bacteriol., 193, 4904-4913.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.8.1.5

Protein Variants

Protein Variants Comment Organism
C82A mutagenesis of the interchange thiol, abolishes all redox-dependent reactions. Redox-independent acetoacetate decarboxylation is not decreased Xanthobacter autotrophicus
C87A mutagenesis of the flavin thiol, results in an inactive enzyme for steady-state redox-dependent reactions, but this variant catalyzes a single-turnover reaction producing a 0.8:1 ratio of product to enzyme. Redox-independent acetoacetate decarboxylation is not decreased Xanthobacter autotrophicus
H137A mutagenesis of the histidine proximal to the ordered water molecule, leads to nearly complete loss of redox-dependent reactions. Redox-independent acetoacetate decarboxylation is not decreased Xanthobacter autotrophicus
H84A mutagenesis of the distal histidine residue, reduces the redox-dependent activities by 58 to 76%. Redox-independent acetoacetate decarboxylation is not decreased Xanthobacter autotrophicus
M140A residue flanking the substrate, catalytic efficiency for 2-(2-oxopropylthio)ethanesulfonate carboxylation is 47fold lower than that for wild-type Xanthobacter autotrophicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.42
-
 2-(2-oxopropylthio)ethanesulfonate wild-type, pH 7.4, 30°C Xanthobacter autotrophicus
6.37
-
 2-(2-oxopropylthio)ethanesulfonate mutant M140A, pH 7.4, 30°C Xanthobacter autotrophicus

Organism

Organism UniProt Comment Textmining
Xanthobacter autotrophicus Q56839
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-(2-oxopropylthio)ethanesulfonate + CO2 + NADPH + H+
-
 Xanthobacter autotrophicus 2-mercaptoethanesulfonate + acetoacetate + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
xecC
-
 Xanthobacter autotrophicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.072
-
 2-(2-oxopropylthio)ethanesulfonate mutant M140A, pH 7.4, 30°C Xanthobacter autotrophicus
0.22
-
 2-(2-oxopropylthio)ethanesulfonate wild-type, pH 7.4, 30°C Xanthobacter autotrophicus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.011
-
 2-(2-oxopropylthio)ethanesulfonate mutant M140A, pH 7.4, 30°C Xanthobacter autotrophicus
0.53
-
 2-(2-oxopropylthio)ethanesulfonate wild-type, pH 7.4, 30°C Xanthobacter autotrophicus