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Literature summary for 1.8.1.7 extracted from
- Purification and characterization of glutathione reductase encoded by a cloned and over-expressed gene in Escherichia coli (1987), Biochem. J., 245, 875-880.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| Escherichia coli gene | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.025 | - |
NADPH | - |
Escherichia coli |  |
| 0.07 | - |
GSSG | - |
Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 49000 | - |
2 * 49000, SDS-PAGE | Escherichia coli |
| 94000 | - |
gel filtration | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| enzyme expressed from plasmid, small and large scale | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 334 | - |
recombinant additional enzyme in Escherichia coli | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GSSG + NADPH | - |
Escherichia coli | glutathione + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 49000, SDS-PAGE | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Escherichia coli | |
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