General Stability | Organism |
---|---|
complete inactivation after incubation with NADPH, reversible by addition of GSSG | Spinacia oleracea |
glutathione stabilizes chloroplast enzymes | Spinacia oleracea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glutathione | product inhibition | Spinacia oleracea | |
iodoacetate | - |
Spinacia oleracea | |
N-ethylmaleimide | - |
Spinacia oleracea | |
NADP+ | competitive against NADPH | Spinacia oleracea | |
Zn2+ | - |
Spinacia oleracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
NADPH | chloroplast enzyme | Spinacia oleracea | |
0.21 | - |
GSSG | - |
Spinacia oleracea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | stroma | Spinacia oleracea | 9507 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72000 | - |
2 * 72000, SDS-PAGE | Spinacia oleracea |
145000 | - |
gel filtration | Spinacia oleracea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spinacia oleracea | - |
spinach | - |
Purification (Comment) | Organism |
---|---|
- |
Spinacia oleracea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Spinacia oleracea | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
246 | - |
- |
Spinacia oleracea |
Storage Stability | Organism |
---|---|
4°C, several weeks | Spinacia oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GSSG + NADPH | reverse reaction only if very high concentration of NADP+ and glutathione present | Spinacia oleracea | glutathione + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 72000, SDS-PAGE | Spinacia oleracea |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
10 min, complete loss of activity | Spinacia oleracea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9 | - |
Spinacia oleracea |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9.5 | pH 7: about 40% of activity maximum, pH 9.5: about 55% of activity maximum | Spinacia oleracea |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.6 | - |
30 min, stable | Spinacia oleracea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | low activity with NADH | Spinacia oleracea | |
NADPH | - |
Spinacia oleracea |