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Literature summary for 1.8.1.7 extracted from

  • Cunningham, M.L.; Zvelebil, M.J.J.M.; Fairlamb, A.H.
    Mechanism of inhibition of trypanothione reductase and glutathione reductase by trivalent organic arsenicals (1994), Eur. J. Biochem., 221, 285-295.
    View publication on PubMed
Search for more literature for 1.8.1.7

Activating Compound

Activating Compound Comment Organism Structure
FAD analogues
-
 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Melarsen oxide 2-step process Homo sapiens
Melarsen oxide i.e. p-(4,6-diamono-s-triazin-2-yl)aminophenylarsenoxide; potent inhibitor, reversible by excess of thiols, more sensitive with NADPH Trypanosoma brucei gambiense
Melarsen oxide i.e. p-(4,6-diamono-s-triazin-2-yl)aminophenylarsenoxide; potent inhibitor, reversible by excess of thiols, more sensitive with NADPH Trypanosoma brucei rhodesiense
NADPH protection by NADP+ Homo sapiens

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Homo sapiens
-
-
-
Trypanosoma brucei gambiense
-
 parasitic protozoa
-
Trypanosoma brucei rhodesiense
-
 parasitic protozoa
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens
-
 Bos taurus

Reaction

Reaction Comment Organism Reaction ID
2 glutathione + NADP+ = glutathione disulfide + NADPH + H+ inhibition mechanism Homo sapiens
a
2 glutathione + NADP+ = glutathione disulfide + NADPH + H+ analogous to trypanothione reductase Trypanosoma brucei rhodesiense
a
2 glutathione + NADP+ = glutathione disulfide + NADPH + H+ analogous to trypanothione reductase Trypanosoma brucei gambiense
a

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
 Homo sapiens
-
intestine mucosa Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
114
-
 purified enzyme Bos taurus
262
-
 purified enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GSSG + NADPH
-
 Homo sapiens glutathione + NADP+
-
 ?
GSSG + NADPH
-
 Bos taurus glutathione + NADP+
-
 ?
GSSG + NADPH
-
 Trypanosoma brucei rhodesiense glutathione + NADP+
-
 ?
GSSG + NADPH
-
 Trypanosoma brucei gambiense glutathione + NADP+
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
27
-
 assay at Homo sapiens
27
-
 assay at Bos taurus
27
-
 assay at Trypanosoma brucei rhodesiense
27
-
 assay at Trypanosoma brucei gambiense

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
 assay at Homo sapiens
7.8
-
 assay at Bos taurus

Cofactor

Cofactor Comment Organism Structure
FAD FAD enzyme Homo sapiens
FAD FAD enzyme Bos taurus
FAD FAD enzyme Trypanosoma brucei rhodesiense
FAD FAD enzyme Trypanosoma brucei gambiense