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Literature summary for 1.8.1.7 extracted from
- Optimized heterologous expression of glutathione reductase from cyanobacterium Anabaena PCC 7120 and characterization of the recombinant protein (1999), Protein Expr. Purif., 15, 92-98.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli of a His-tagged high-level expression mutant | Anabaena sp. |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | introduction of silent random mutations in 5'-region to generate a His-tagged high-level expression clone | Anabaena sp. |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.16 | - |
GSSG | recombinant enzyme | Anabaena sp. |  |
| 0.24 | - |
NADPH | recombinant enzyme | Anabaena sp. | |
| 1.12 | - |
NADH | recombinant enzyme | Anabaena sp. | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GSSG + NADPH | Anabaena sp. | high intracellular concentration of glutathione is important for functionality of thiol groups of cellular proteins | glutathione + NADP+ | - |
ir | |
| GSSG + NADPH | Anabaena sp. | serves in detoxification | glutathione + NADP+ | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Anabaena sp. | - |
PCC 7120, cyanobacterium | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| via high-level expression of recombinant enzyme in Escherichia coli | Anabaena sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 246 | - |
recombinant purified enzyme | Anabaena sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione disulfide + NADH + H+ | - |
Anabaena sp. | glutathione + NAD+ | - |
? | |
| GSSG + NADPH | - |
Anabaena sp. | glutathione + NADP+ | - |
ir | |
| GSSG + NADPH | high intracellular concentration of glutathione is important for functionality of thiol groups of cellular proteins | Anabaena sp. | glutathione + NADP+ | - |
ir | |
| GSSG + NADPH | serves in detoxification | Anabaena sp. | glutathione + NADP+ | - |
ir | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GR | - |
Anabaena sp. |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 38 | - |
NADH | recombinant enzyme | Anabaena sp. | |
| 367 | - |
NADPH | recombinant enzyme | Anabaena sp. | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
NADPH + GSSG | Anabaena sp. |
| 8 | - |
NADH + GSSG | Anabaena sp. |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | low activity with NADH | Anabaena sp. | |
| NADPH | can utilize both NADPH and NADH, more active with NADPH | Anabaena sp. | |
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Release 2023.1 (January 2023)
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