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Literature summary for 1.8.1.7 extracted from

  • Knapp, K.G.; Swartz, J.R.
    Cell-free production of active E. coli thioredoxin reductase and glutathione reductase (2004), FEBS Lett., 559, 66-70.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
in-vitro His-tagged enzyme expression Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information cell-free enzyme synthesis, 71% of which is soluble, the enzyme is dimeric, requires FAD as a cofactor, and is more active than the native enzyme Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glutathione disulfide + NADPH Escherichia coli key enzyme in maintaining the reducing environment in the cell, maintainance of sulfhydryl redox potential in the cytoplasm glutathione + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from in-vitro expression by nickel chelate affinity chromatography Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Escherichia coli
139
-
purified in vitro-synthesized enzyme, 30°C, 0.002 mM FAD Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione disulfide + NADPH
-
Escherichia coli glutathione + NADP+
-
?
glutathione disulfide + NADPH key enzyme in maintaining the reducing environment in the cell, maintainance of sulfhydryl redox potential in the cytoplasm Escherichia coli glutathione + NADP+
-
?

Subunits

Subunits Comment Organism
dimer homodimer Escherichia coli

Synonyms

Synonyms Comment Organism
glutathione reductase
-
Escherichia coli
GR
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Escherichia coli

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 37 increasing activity between 25°C and 37°C Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.1
-
assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
FAD required for activity Escherichia coli
NADPH
-
Escherichia coli