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Literature summary for 1.8.1.7 extracted from
- Crystal structure of glutathione reductase Glr1 from the yeast Saccharomyces cerevisiae (2007), Proteins Struct. Funct. Bioinform., 68, 972-979.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression of Saccharomyces cerevisiae Glr1 in Pichia pastoris GS115 | Saccharomyces cerevisiae |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging drop method, crystal structure is determined at 2.40 A resolution | Saccharomyces cerevisiae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | N278 at the vicinity of NADP-binding pocket is artificially glycosylated when heterogeneously overexpressed in Pichia pastoris. The highly motile oligosaccharide chain linked to N278 of the recombinant Glr1 interferes with the entry of NADPH, wich results in a dramatic increase of Km for NAPDH and a significant decrease of turnover number, when compared with the native protein | Saccharomyces cerevisiae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.015 | - |
NADPH | native Glr1 | Saccharomyces cerevisiae |  |
| 0.0323 | - |
NADPH | recombinant Glr1 | Saccharomyces cerevisiae | |
| 0.0746 | - |
GSSG | native Glr1 | Saccharomyces cerevisiae | |
| 0.2625 | - |
GSSG | recombinant Glr1 | Saccharomyces cerevisiae | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | P41921 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GSSG + NADPH + H+ | - |
Saccharomyces cerevisiae | glutathione + NADP+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glutathione reductase Glr1 | - |
Saccharomyces cerevisiae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 463.3 | - |
NADPH | recombinant Glr1 | Saccharomyces cerevisiae | |
| 900 | - |
NADPH | native Glr1 | Saccharomyces cerevisiae | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Saccharomyces cerevisiae | |
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Release 2023.1 (January 2023)
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