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Literature summary for 1.8.1.7 extracted from

  • Ding, S.; Lu, Q.; Zhang, Y.; Yang, Z.; Wen, X.; Zhang, L.; Lu, C.
    Enhanced sensitivity to oxidative stress in transgenic tobacco plants with decreased glutathione reductase activity leads to a decrease in ascorbate pool and ascorbate redox state (2009), Plant Mol. Biol., 69, 577-592.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Nicotiana tabaccum cultivar Wisconsin 38 Nicotiana tabacum

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Nicotiana tabacum 9507
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
one of two minor protein bands at 50000 and 47000 Da, SDS-PAGE Nicotiana tabacum
50000
-
one of two minor protein bands at 50000 and 47000 Da, SDS-PAGE Nicotiana tabacum
55000
-
major protein band, approximately 55000 Da, SDS-PAGE Nicotiana tabacum

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum P80461
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose resin column chromatography Nicotiana tabacum

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Nicotiana tabacum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione disulfide + NADPH + H+
-
Nicotiana tabacum glutathione + NADP+
-
?

Synonyms

Synonyms Comment Organism
glutathione reductase
-
Nicotiana tabacum

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Nicotiana tabacum

Expression

Organism Comment Expression
Nicotiana tabacum after treatment with methyl viologen for two weeks, there is a significant increase in the glutathione reductase activity in both wild type and transgenic plants and such an increase is greater in transgenic plants than in wild type plants up