KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0153 | - |
NADPH | in 100 mM potassium phosphate buffer, pH 7.4, 4 mM EDTA, at 37°C | Rattus norvegicus | |
0.0531 | - |
glutathione disulfide | in 100 mM potassium phosphate buffer, pH 7.4, 4 mM EDTA, at 37°C | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
native PAGE and SDS-PAGE | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
heat-denaturation and Sephadex G25 gel filtration, 2',5'-ADP agarose 4B column chromatography, and PBE94 column chromatography | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.11 | - |
crude liver homogenate, in 100 mM potassium phosphate buffer, pH 7.4, 4 mM EDTA, at 37°C | Rattus norvegicus |
250 | - |
after 2356fold purification, in 100 mM potassium phosphate buffer, pH 7.4, 4 mM EDTA, at 37°C | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione disulfide + NADH + H+ | NADH is used as a coenzyme with a lower efficiency (32.7%) than NADPH | Rattus norvegicus | glutathione + NAD+ | - |
? | |
glutathione disulfide + NADPH + H+ | - |
Rattus norvegicus | glutathione + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glutathione reductase | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | each mol of enzyme subunit binds 0.97 mol of FAD | Rattus norvegicus | |
NADH | NADH is used as a coenzyme by rat kidney glutathione reductase but with a lower efficiency (32.7%) than NADPH | Rattus norvegicus | |
NADPH | - |
Rattus norvegicus |
General Information | Comment | Organism |
---|---|---|
physiological function | glutathione reductase is the crucial enzyme to maintain high glutathione/glutathione disulfide ratio and physiological redox status in cells | Rattus norvegicus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4850 | - |
glutathione disulfide | in 100 mM potassium phosphate buffer, pH 7.4, 4 mM EDTA, at 37°C | Rattus norvegicus | |
16800 | - |
NADPH | in 100 mM potassium phosphate buffer, pH 7.4, 4 mM EDTA, at 37°C | Rattus norvegicus |