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Literature summary for 1.8.1.7 extracted from
- Identification and biochemical characterization of the glutathione reductase family from Populus trichocarpa (2020), Plant Sci., 294, 110459 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Populus trichocarpa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | - |
Populus trichocarpa |  |
| Cu2+ | - |
Populus trichocarpa | |
| Pb2+ | - |
Populus trichocarpa | |
| Zn2+ | - |
Populus trichocarpa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00171 | - |
glutathione disulfide | enzyme variant GR1.2, pH 7.6, 22°C | Populus trichocarpa | |
| 0.00601 | - |
NADPH | enzyme variant GR2, pH 7.6, 22°C | Populus trichocarpa | |
| 0.01546 | - |
glutathione disulfide | enzyme variant GR1.1, pH 7.6, 22°C | Populus trichocarpa | |
| 0.02662 | - |
NADPH | enzyme variant GR1.2, pH 7.6, 22°C | Populus trichocarpa | |
| 0.03843 | - |
NADPH | enzyme variant GR1.1, pH 7.6, 22°C | Populus trichocarpa | |
| 0.06202 | - |
glutathione disulfide | enzyme variant GR2, pH 7.6, 22°C | Populus trichocarpa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | enzyme variant PtGR2 | Populus trichocarpa | 9507 | - |
| cytoplasm | enzyme variant PtGR1.2 | Populus trichocarpa | 5737 | - |
| cytoplasm | enzyme variants PtGR1.1 | Populus trichocarpa | 5737 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione disulfide + NADPH + H+ | Populus trichocarpa | - |
2 glutathione + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Populus trichocarpa | A0A2K1XHK8 | - |
- |
| Populus trichocarpa | A0A2K2BSL2 | - |
- |
| Populus trichocarpa | A9PHY8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Populus trichocarpa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| bud | - |
Populus trichocarpa | - |
| leaf | - |
Populus trichocarpa | - |
| leaf | PtGR2 has a significantly high level in leaf indicating that PtGR2 might have a very pivotal role in leaves | Populus trichocarpa | - |
| root | - |
Populus trichocarpa | - |
| stem | - |
Populus trichocarpa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione disulfide + NADPH + H+ | - |
Populus trichocarpa | 2 glutathione + NADP+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glutathione reductase | - |
Populus trichocarpa |
| PtGR1.1 | - |
Populus trichocarpa |
| PtGR1.2 | - |
Populus trichocarpa |
| PtGR2 | - |
Populus trichocarpa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 9.94 | - |
glutathione disulfide | enzyme variant GR1.2, pH 7.6, 22°C | Populus trichocarpa | |
| 13.55 | - |
NADPH | enzyme variant GR2, pH 7.6, 22°C | Populus trichocarpa | |
| 14.08 | - |
NADPH | enzyme variant GR1.2, pH 7.6, 22°C | Populus trichocarpa | |
| 19.57 | - |
glutathione disulfide | enzyme variant GR2, pH 7.6, 22°C | Populus trichocarpa | |
| 23.43 | - |
glutathione disulfide | enzyme variant GR1.1, pH 7.6, 22°C | Populus trichocarpa | |
| 29.28 | - |
NADPH | enzyme variant GR1.1, pH 7.6, 22°C | Populus trichocarpa | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
enzyme variant PtGR1.1 | Populus trichocarpa |
| 8 | - |
enzyme variant PtGR1.2 | Populus trichocarpa |
| 8 | - |
enzyme variant PtGR2 | Populus trichocarpa |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Populus trichocarpa | expression of PtGR genes is general upregulated under salicylic acid and alamethicin treatment | up |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 225 | - |
NADPH | enzyme variant GR2, pH 7.6, 22°C | Populus trichocarpa | |
| 320 | - |
glutathione disulfide | enzyme variant GR2, pH 7.6, 22°C | Populus trichocarpa | |
| 530 | - |
NADPH | enzyme variant GR1.2, pH 7.6, 22°C | Populus trichocarpa | |
| 760 | - |
NADPH | enzyme variant GR1.1, pH 7.6, 22°C | Populus trichocarpa | |
| 1520 | - |
glutathione disulfide | enzyme variant GR1.1, pH 7.6, 22°C | Populus trichocarpa | |
| 5790 | - |
glutathione disulfide | enzyme variant GR1.2, pH 7.6, 22°C | Populus trichocarpa | |
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