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Literature summary for 1.8.1.9 extracted from
- Reaction of both active site thiols of reduced thioredoxin reductase with N-ethylmaleimide (1985), Biochemistry, 24, 7617-7621.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cysteine | part of the active site | Escherichia coli |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| N-ethylmaleimide | reaction only with the reduced enzyme | Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| thioredoxin + NADP+ = thioredoxin disulfide + NADPH + H+ | inhibition mechanism | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH | i.e. DTNB | Escherichia coli | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
| 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH | requires thioredoxin for reduction of DTNB | Escherichia coli | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
| 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH | coupled assay | Escherichia coli | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
| thioredoxin + NADP+ | - |
Escherichia coli | thioredoxin disulfide + NADPH | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Escherichia coli | |
| NADPH | - |
Escherichia coli | |
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