Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoate) | above 0.1 mM | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
2 * 58000, SDS-PAGE | Homo sapiens |
105000 | - |
gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
HeLa cells | - |
Purification (Comment) | Organism |
---|---|
HeLa cells, partially | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HeLa cell | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
42 | - |
purified enzyme, coupled assay with DTNB | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH | i.e. DTNB | Homo sapiens | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
thioredoxin + NADP+ | coupled assay with DTNB | Homo sapiens | thioredoxin disulfide + NADPH | - |
? | |
thioredoxin + NADP+ | coupled assay with adenosine 3'-phosphate 5'-phosphosulfate reductase | Homo sapiens | thioredoxin disulfide + NADPH | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 58000, SDS-PAGE | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Homo sapiens | |
NADPH | - |
Homo sapiens |