Literature summary for 1.8.1.9 extracted from

Jacquot, J.P.; Rivera-Madrid, R.; Marinho, P.; Kollarova, M.; Le Marechal, P.; Miginiac-Maslow, M.; Meyer, Y.
Arabidopsis thaliana NADPH thioredoxin reductase. cDNA characterization and expression of the recombinant protein in Escherichia coli (1994), J. Mol. Biol., 235, 1357-1363.

Search for more literature for 1.8.1.9

Activating Compound

Activating Compound	Comment	Organism	Structure
cysteine	part of the active site	Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment)	Organism
strong overexpression in Escherichia coli, DNA sequence analysis	Arabidopsis thaliana

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	additionally to the Arabidopsis thaliana thioredoxin, several other thioredoxins have been tested, Km-values	Arabidopsis thaliana
0.0011	-	thioredoxin	recombinant enzyme	Arabidopsis thaliana

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Arabidopsis thaliana	5829	-

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	-	EMBL accession numbers Z23109, Z23108	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant from Escherichia coli	Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
thioredoxin + NADP+	-	Arabidopsis thaliana	thioredoxin disulfide + NADPH	-	?
thioredoxin + NADP+	i.e. DTNB	Arabidopsis thaliana	thioredoxin disulfide + NADPH + H+	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 35000-35328, SDS-PAGE and amino acid analysis	Arabidopsis thaliana

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
60	-	5 min, active	Arabidopsis thaliana

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Arabidopsis thaliana
NADPH	specific for	Arabidopsis thaliana

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)