Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-Bromopropionate | - |
Homo sapiens | |
arsenite | - |
Homo sapiens | |
iodoacetate | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0037 | - |
thioredoxin | thioredoxin from rat | Homo sapiens | |
0.034 | - |
thioredoxin | thioredoxin from E. coli | Homo sapiens | |
0.116 | - |
DTNB | - |
Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
selenocysteine | contains selenocysteine as part of the redox active selenosulfide in the active center | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
2 * 57000, SDS-PAGE | Homo sapiens |
110000 | - |
gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | no glycosyl groups on the protein | Homo sapiens |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
lung | adenocarcinoma cell line NCI-H441 | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
31 | - |
DTNB coupled assay, purified enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH | i.e. DTNB | Homo sapiens | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH | coupled assay | Homo sapiens | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
GSSG + NADPH | - |
Homo sapiens | GSH + NADP+ | - |
? | |
menadione + NADPH | - |
Homo sapiens | ? | - |
? | |
oxidized lipoate + NADPH | - |
Homo sapiens | ? | - |
? | |
rat thioredoxin + NADP+ | - |
Homo sapiens | rat thioredoxin disulfide + NADPH + H+ | - |
r | |
thioredoxin + NADP+ | coupled assay with DTNB | Homo sapiens | thioredoxin disulfide + NADPH | - |
? | |
thioredoxin + NADP+ | coupled assay, measurement of NADPH oxidation in presence of insulin and thioredoxin | Homo sapiens | thioredoxin disulfide + NADPH | - |
? | |
thioredoxin + NADP+ | - |
Homo sapiens | thioredoxin disulfide + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 57000, SDS-PAGE | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.83 | - |
rat thioredoxin | insulin coupled assay | Homo sapiens | |
5.58 | - |
thioredoxin | insulin coupled assay method | Homo sapiens | |
29.5 | - |
DTNB | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Homo sapiens | |
NADPH | - |
Homo sapiens |