Cloned (Comment) | Organism |
---|---|
overexpression of wild-type and mutant enzymes from plasmids | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | trxB gene is combined to chimeric enzyme NT-TrR by exchanging the N-terminus of Escherichia coli with the N-terminus of Salmonella typhimurium AhpF gene protein, which encodes a protein with about 35% homology in the N-terminal region, 2 other mutants are constructed in the same way but with double mutation C129S/C132S and C342S/C345S, the first in the Escherichia coli part and the latter in the Salmonella part of the chimeric protein, activity corressponding to organism wild-type giving the N-terminal part, except for C342S/C345S chimeric mutant who has no activity | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
wild-type and chimeric mutants with and without amino acid exchanges | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
thioredoxin + NADP+ | coupled assay with DTNB | Escherichia coli | thioredoxin disulfide + NADPH | - |
? | |
thioredoxin + NADP+ | coupled assay, measurement of NADPH oxidation in presence of insulin and thioredoxin | Escherichia coli | thioredoxin disulfide + NADPH | - |
? | |
thioredoxin + NADP+ | NADH in the standard assay, wild-type and chimeric mutants with and without amino acid exchanges | Escherichia coli | thioredoxin disulfide + NADPH | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TrR | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | wild-type and recombinant chimeric mutants in a coupled assay | Escherichia coli | |
50.3 | - |
DTNB | chimeric enzyme mutant, partly from Salmonella typhimurium AhpF protein | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | wild-type and chimeric mutants partly from Salmonella typhimurium AhpF protein | Escherichia coli | |
NADH | wild-type and mutants | Escherichia coli | |
NADPH | wild-type and mutants | Escherichia coli |