Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Aeropyrum pernix |
General Stability | Organism |
---|---|
FAD increases thermostability | Aeropyrum pernix |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.6 | - |
NADPH | - |
Aeropyrum pernix | |
12.3 | - |
thioredoxin | - |
Aeropyrum pernix | |
172.4 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | - |
Aeropyrum pernix |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75000 | - |
gel filtration | Aeropyrum pernix |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aeropyrum pernix | - |
K1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH | - |
Aeropyrum pernix | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
thioredoxin disulfide + NADPH | - |
Aeropyrum pernix | thioredoxin + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Aeropyrum pernix |
Synonyms | Comment | Organism |
---|---|---|
ApTR | - |
Aeropyrum pernix |
thioredoxin reductase | - |
Aeropyrum pernix |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
above | Aeropyrum pernix |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 95 | 70°C: about 50% of maximal activity, 95°C: about 95% of maximal activity | Aeropyrum pernix |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
110 | - |
half-life: 4 min | Aeropyrum pernix |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | - |
Aeropyrum pernix | |
63.2 | - |
thioredoxin | - |
Aeropyrum pernix |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Aeropyrum pernix | |
NADPH | - |
Aeropyrum pernix |