Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta (DE3) cells | Hordeum vulgare |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 24% (w/v) PEG 400, 2% Jeffamine M-600, and 0.1 M citrate buffer pH 3.5 | Hordeum vulgare |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hordeum vulgare | A9LN30 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap HP affinity column chromatography, gel filtration | Hordeum vulgare |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
thioredoxin disulfide + NADPH + H+ | - |
Hordeum vulgare | thioredoxin + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | x-ray crystallography | Hordeum vulgare |
Synonyms | Comment | Organism |
---|---|---|
NADPH-dependent thioredoxin reductase 2 | - |
Hordeum vulgare |
NTR2 | - |
Hordeum vulgare |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | contains one FAD molecule per subunit | Hordeum vulgare | |
NADPH | dependent on | Hordeum vulgare |