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Literature summary for 1.8.1.9 extracted from

  • Kirkensgaard, K.G.; Haegglund, P.; Finnie, C.; Svensson, B.; Henriksen, A.
    Structure of Hordeum vulgare NADPH-dependent thioredoxin reductase 2. Unwinding the reaction mechanism (2009), Acta Crystallogr. Sect. D, 65, 932-941.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta (DE3) cells Hordeum vulgare

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 24% (w/v) PEG 400, 2% Jeffamine M-600, and 0.1 M citrate buffer pH 3.5 Hordeum vulgare

Organism

Organism UniProt Comment Textmining
Hordeum vulgare A9LN30
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap HP affinity column chromatography, gel filtration Hordeum vulgare

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
thioredoxin disulfide + NADPH + H+
-
Hordeum vulgare thioredoxin + NADP+
-
?

Subunits

Subunits Comment Organism
homodimer x-ray crystallography Hordeum vulgare

Synonyms

Synonyms Comment Organism
NADPH-dependent thioredoxin reductase 2
-
Hordeum vulgare
NTR2
-
Hordeum vulgare

Cofactor

Cofactor Comment Organism Structure
FAD contains one FAD molecule per subunit Hordeum vulgare
NADPH dependent on Hordeum vulgare