Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Rattus norvegicus |
Crystallization (Comment) | Organism |
---|---|
in the presence of 10 mM NADP+, hanging drop vapor diffusion method, using 15% polyethylene glycol 3350, 12% ethylene glycol, and 0.1 M HEPES, pH 7.5 | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
Y116F | the mutant protein is not soluble | Rattus norvegicus |
Y116I | the mutation decreases sensitivity to inhibition by cisplatin and lowers catalytic efficiency in reduction of thioredoxin compared to the wild type enzyme | Rattus norvegicus |
Y116I | the mutation decreases sensitivity to inhibition by cisplatin, lowers catalytic efficiency in reduction of thioredoxin, and increases turnover using 5-hydroxy-1,4-naphthoquinone (juglone) as substrate compared to the wild type enzyme | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cisplatin | complete inhibition after 1 h at 0.5 mM | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00099 | - |
thioredoxin disulfide | mutant enzyme Y116I, in TE buffer, at 20°C | Rattus norvegicus | |
0.00106 | - |
thioredoxin disulfide | mutant enzyme Y116T, in TE buffer, at 20°C | Rattus norvegicus | |
0.00143 | - |
thioredoxin disulfide | wild type enzyme, in TE buffer, at 20°C | Rattus norvegicus | |
0.00227 | - |
5-hydroxy-1,4-naphthoquinone | wild type enzyme, in TE buffer, at 20°C | Rattus norvegicus | |
0.00698 | - |
5-hydroxy-1,4-naphthoquinone | mutant enzyme Y116T, in TE buffer, at 20°C | Rattus norvegicus | |
0.02374 | - |
5-hydroxy-1,4-naphthoquinone | mutant enzyme Y116I, in TE buffer, at 20°C | Rattus norvegicus | |
0.0906 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | mutant enzyme Y116T, in TE buffer, at 20°C | Rattus norvegicus | |
0.094 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | wild type enzyme, in TE buffer, at 20°C | Rattus norvegicus | |
0.0971 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | mutant enzyme Y116I, in TE buffer, at 20°C | Rattus norvegicus | |
2.89 | - |
Lipoamide | wild type enzyme, in TE buffer, at 20°C | Rattus norvegicus | |
3.68 | - |
Lipoamide | mutant enzyme Y116I, in TE buffer, at 20°C | Rattus norvegicus | |
5.59 | - |
Lipoamide | mutant enzyme Y116T, in TE buffer, at 20°C | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
selenium | thioredoxin reductase 1 is a selenoprotein, the oxidized enzyme presents a selenenylsulfide motif in trans-configuration, with the selenium atom of selenocysteine-498 positioned beneath the side chain of Tyr-116 | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | O89049 | - |
- |
Purification (Comment) | Organism |
---|---|
2',5'-ADP Sepharose column chromatography and phenylarsine oxide-Sepharose column chroamtography | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+ | - |
Rattus norvegicus | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
5-hydroxy-1,4-naphthoquinone + NADPH + H+ | - |
Rattus norvegicus | ? | - |
? | |
lipoamide + NADPH + H+ | - |
Rattus norvegicus | dihydrolipoamide + NADP+ | - |
? | |
thioredoxin + insulin disulfide | thioredixin is the native principal substrate of TrxR1 | Rattus norvegicus | thioredoxin disulfide + insulin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
thioredoxin reductase 1 | - |
Rattus norvegicus |
TrxR1 | - |
Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | 3.7 | Lipoamide | wild type enzyme, in TE buffer, at 20°C | Rattus norvegicus | |
24.1 | - |
5-hydroxy-1,4-naphthoquinone | wild type enzyme, in TE buffer, at 20°C | Rattus norvegicus | |
27.6 | - |
Lipoamide | mutant enzyme Y116I, in TE buffer, at 20°C | Rattus norvegicus | |
31.2 | - |
Lipoamide | mutant enzyme Y116T, in TE buffer, at 20°C | Rattus norvegicus | |
40.98 | - |
thioredoxin disulfide | mutant enzyme Y116I, in TE buffer, at 20°C | Rattus norvegicus | |
44.85 | - |
thioredoxin disulfide | mutant enzyme Y116T, in TE buffer, at 20°C | Rattus norvegicus | |
47.72 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | mutant enzyme Y116T, in TE buffer, at 20°C | Rattus norvegicus | |
49.87 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | mutant enzyme Y116I, in TE buffer, at 20°C | Rattus norvegicus | |
52.75 | - |
5-hydroxy-1,4-naphthoquinone | mutant enzyme Y116T, in TE buffer, at 20°C | Rattus norvegicus | |
70.3 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | wild type enzyme, in TE buffer, at 20°C | Rattus norvegicus | |
94.17 | - |
thioredoxin disulfide | wild type enzyme, in TE buffer, at 20°C | Rattus norvegicus | |
174.3 | - |
5-hydroxy-1,4-naphthoquinone | mutant enzyme Y116I, in TE buffer, at 20°C | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | contains 6 FAD molecules | Rattus norvegicus | |
NADP+ | contains 6 NADP+ molecules | Rattus norvegicus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.6 | - |
Lipoamide | mutant enzyme Y116T, in TE buffer, at 20°C | Rattus norvegicus | |
7.5 | - |
Lipoamide | mutant enzyme Y116I, in TE buffer, at 20°C | Rattus norvegicus | |
513.3 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | mutant enzyme Y116I, in TE buffer, at 20°C | Rattus norvegicus | |
526.7 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | mutant enzyme Y116T, in TE buffer, at 20°C | Rattus norvegicus | |
748.3 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | wild type enzyme, in TE buffer, at 20°C | Rattus norvegicus | |
7343 | - |
5-hydroxy-1,4-naphthoquinone | mutant enzyme Y116I, in TE buffer, at 20°C | Rattus norvegicus | |
7557 | - |
5-hydroxy-1,4-naphthoquinone | mutant enzyme Y116T, in TE buffer, at 20°C | Rattus norvegicus | |
10620 | - |
5-hydroxy-1,4-naphthoquinone | wild type enzyme, in TE buffer, at 20°C | Rattus norvegicus | |
41450 | - |
thioredoxin disulfide | mutant enzyme Y116I, in TE buffer, at 20°C | Rattus norvegicus | |
42150 | - |
thioredoxin disulfide | mutant enzyme Y116T, in TE buffer, at 20°C | Rattus norvegicus | |
65940 | - |
thioredoxin disulfide | wild type enzyme, in TE buffer, at 20°C | Rattus norvegicus |