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Literature summary for 1.8.1.9 extracted from

  • Seo, H.J.; Lee, Y.N.
    Characterization of Deinococcus radiophilus thioredoxin reductase active with both NADH and NADPH (2010), J. Microbiol., 48, 637-643.
    View publication on PubMed
Search for more literature for 1.8.1.9

Inhibitors

Inhibitors Comment Organism Structure
4-azobenzene sulfonic acid 63% residual activity at 5 mM Deinococcus radiophilus
Ag+ enzyme activity is moderately reduced (50%) by 1 mM Deinococcus radiophilus
Cd2+ enzyme activity is drastically reduced (70%) by 1 mM Deinococcus radiophilus
Cu2+ enzyme activity is drastically reduced (70%) by 1 mM Deinococcus radiophilus
Glyoxal 93% residual activity at 5 mM Deinococcus radiophilus
Hg2+ enzyme activity is drastically reduced (70%) by 1 mM Deinococcus radiophilus
Iodine 86% residual activity at 5 mM Deinococcus radiophilus
additional information not inhibited by 1-chloro-2,4-dinitrobenzene Deinococcus radiophilus
N-ethylmaleimide 46% residual activity at 5 mM Deinococcus radiophilus
Zn2+ enzyme activity is drastically reduced (70%) by 1 mM Deinococcus radiophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0125
-
 NADPH in 50 mM potassium phosphate buffer (pH 7.0), at 22°C Deinococcus radiophilus
0.0302
-
 NADH in 50 mM potassium phosphate buffer (pH 7.0), at 22°C Deinococcus radiophilus
0.463
-
 5,5'-dithiobis(2-nitrobenzoic acid) in 50 mM potassium phosphate buffer (pH 7.0), at 22°C Deinococcus radiophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63100
-
 PAGE Deinococcus radiophilus
72200
-
 gel filtration Deinococcus radiophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
thioredoxin disulfide + NADH + H+ Deinococcus radiophilus
-
 thioredoxin + NAD+
-
 ?
thioredoxin disulfide + NADH + H+ Deinococcus radiophilus ATCC 27603
-
 thioredoxin + NAD+
-
 ?
thioredoxin disulfide + NADPH + H+ Deinococcus radiophilus
-
 thioredoxin + NADP+
-
 ?
thioredoxin disulfide + NADPH + H+ Deinococcus radiophilus ATCC 27603
-
 thioredoxin + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Deinococcus radiophilus
-
-
-
Deinococcus radiophilus ATCC 27603
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, 2'5'-ADP Sepharose 4B affinity column chromatography, and Sephadex G-100 gel filtration Deinococcus radiophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.77
-
 enzyme from cell-free extract, with NADPH, in 50 mM potassium phosphate buffer (pH 7.0), at 22°C Deinococcus radiophilus
367.7
-
 after 478fold purification, with NADPH, in 50 mM potassium phosphate buffer (pH 7.0), at 22°C Deinococcus radiophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,5'-dithiobis(2-nitrobenzoic acid) + NADH + H+
-
 Deinococcus radiophilus 5'-thionitrobenzoic acid + NAD+
-
 ?
5,5'-dithiobis(2-nitrobenzoic acid) + NADH + H+
-
 Deinococcus radiophilus ATCC 27603 5'-thionitrobenzoic acid + NAD+
-
 ?
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+
-
 Deinococcus radiophilus 5'-thionitrobenzoic acid + NADP+
-
 ?
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+
-
 Deinococcus radiophilus ATCC 27603 5'-thionitrobenzoic acid + NADP+
-
 ?
thioredoxin disulfide + NADH + H+
-
 Deinococcus radiophilus thioredoxin + NAD+
-
 ?
thioredoxin disulfide + NADH + H+
-
 Deinococcus radiophilus ATCC 27603 thioredoxin + NAD+
-
 ?
thioredoxin disulfide + NADPH + H+
-
 Deinococcus radiophilus thioredoxin + NADP+
-
 ?
thioredoxin disulfide + NADPH + H+
-
 Deinococcus radiophilus ATCC 27603 thioredoxin + NADP+
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 000, SDS-PAGE Deinococcus radiophilus

Synonyms

Synonyms Comment Organism
EC 1.6.4.5 formerly Deinococcus radiophilus
L-TrxR
-
 Deinococcus radiophilus
TrxR
-
 Deinococcus radiophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70 90 the enzyme is stable at temperatures ranging from 4°C to 70°C for 30 min. However, the enzyme activity is drastically reduced at 80°C and completely inactivated at 90°C Deinococcus radiophilus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 9 the purified enzyme is stable at pH 6.0-7.0 and relatively stable even at pH 8.0-9.0, but quite unstable below pH 5.0 Deinococcus radiophilus

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Deinococcus radiophilus
NADH
-
 Deinococcus radiophilus
NADPH
-
 Deinococcus radiophilus

pI Value

Organism Comment pI Value Maximum pI Value
Deinococcus radiophilus isoelectric focusing
-
 4.5