Literature summary for 1.8.1.B1 extracted from

Rendon, J.L.; del Arenal, I.P.; Guevara-Flores, A.; Uribe, A.; Plancarte, A.; Mendoza-Hernandez, G.
Purification, characterization and kinetic properties of the multifunctional thioredoxin-glutathione reductase from Taenia crassiceps metacestode (cysticerci) (2004), Mol. Biochem. Parasitol., 133, 61-69.

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General Stability

General Stability	Organism
continuous freezing and thawing results in loss of the enzyme activity	Taenia crassiceps

Inhibitors

Inhibitors	Comment	Organism	Structure
auranofin	-	Taenia crassiceps

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.003	-	NADPH	pH 7.8, 25°C	Taenia crassiceps
0.015	-	GSSG	pH 7.8, 25°C	Taenia crassiceps
0.017	-	thioredoxin	pH 7.8, 25°C	Taenia crassiceps
0.049	-	5,5'-dithiobis(2-nitrobenzoic acid)	pH 7.8, 25°C	Taenia crassiceps

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
66000	-	1 * 66000, gel electrophoresis under denaturing conditions	Taenia crassiceps
136000	-	gel filtration	Taenia crassiceps

Organism

Organism	UniProt	Comment	Textmining
Taenia crassiceps	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Taenia crassiceps

Source Tissue

Source Tissue	Comment	Organism	Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
4.7	-	GSSG + NADPH	Taenia crassiceps
10.3	-	5,5'-dithiobis(2-nitrobenzoic acid) + NADPH	Taenia crassiceps
17.5	-	thioredoxin disulfide + NADPH	Taenia crassiceps

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+	absolute specificity for NADPH as electron donor	Taenia crassiceps	2-nitro-5-thiobenzoate + NADP+	-	?
GSSG + NADPH + H+	absolute specificity for NADPH as electron donor	Taenia crassiceps	glutathione + NADP+	-	?
thioredoxin disulfide + NADPH	absolute specificity for NADPH as electron donor	Taenia crassiceps	thioredoxin + NADP+	-	?

Subunits

Subunits	Comment	Organism
dimer	1 * 66000, gel electrophoresis under denaturing conditions	Taenia crassiceps

Synonyms

Synonyms	Comment	Organism
thioredoxin-glutathione reductase	-	Taenia crassiceps

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.17	-	5,5'-dithiobis(2-nitrobenzoic acid)	pH 7.8, 25°C	Taenia crassiceps
5.1	-	GSSG	pH 7.8, 25°C	Taenia crassiceps
19.2	-	thioredoxin	pH 7.8, 25°C	Taenia crassiceps

pI Value

Organism	Comment	pI Value Maximum	pI Value
Taenia crassiceps	-	-	5.2

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Release 2023.1 (January 2023)