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Literature summary for 1.8.1.B1 extracted from

  • Guevara-Flores, A.; Del Arenal, I.P.; Mendoza-Hernandez, G.; Pardo, J.P.; Flores-Herrera, O.; Rendon, J.L.
    Mitochondrial thioredoxin-glutathione reductase from larval Taenia crassiceps (Cysticerci) (2010), J. Parasitol. Res., 2010, 2010.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.8.1.B1

Inhibitors

Inhibitors Comment Organism Structure
auranofin complete inhibition at 0.7 nM Taenia crassiceps

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00087
-
 thioredoxin disulfide at pH 7.8 and 25°C Taenia crassiceps
0.019
-
 NADPH at pH 7.8 and 25°C Taenia crassiceps
0.0345
-
 5,5'-dithiobis(2-nitrobenzoic acid) at pH 7.8 and 25°C Taenia crassiceps
0.041
-
 GSSG at pH 7.8 and 25°C Taenia crassiceps

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Taenia crassiceps 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 x * 65000, SDS-PAGE Taenia crassiceps

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GSSG + NADPH + H+ Taenia crassiceps
-
 GSH + NADP+
-
 ?
thioredoxin disulfide + NADPH + H+ Taenia crassiceps
-
 thioredoxin + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Taenia crassiceps
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sephacel gel filtration, hydroxyapatite chromatography column chromatography, and Cibacron blue chromatography column chromatography Taenia crassiceps

Source Tissue

Source Tissue Comment Organism Textmining
larva
-
 Taenia crassiceps
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11.7
-
 crude extract, using GSSG as substrate, at pH 7.8 and 25°C Taenia crassiceps
35
-
 crude extract, using 5,5'-dithiobis(2-nitrobenzoic acid) as substrate, at pH 7.8 and 25°C Taenia crassiceps
128.5
-
 crude extract, using hydroxyethyl disulfide as substrate, at pH 7.8 and 25°C Taenia crassiceps
229
-
 after 31fold purification, using GSSG as substrate, at pH 7.8 and 25°C Taenia crassiceps
1091
-
 after 31fold purification, using 5,5'-dithiobis(2-nitrobenzoic acid) as substrate, at pH 7.8 and 25°C Taenia crassiceps
2243
-
 after 31fold purification, using hydroxyethyl disulfide as substrate, at pH 7.8 and 25°C Taenia crassiceps

Storage Stability

Storage Stability Organism
-20°C, purified enzyme, 15 days, remains stable Taenia crassiceps

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+
-
 Taenia crassiceps 2-nitro-5-thiobenzoate + NADP+
-
 ?
GSSG + NADPH + H+
-
 Taenia crassiceps GSH + NADP+
-
 ?
hydroxyethyl disulfide + NADPH + H+
-
 Taenia crassiceps ?
-
 ?
additional information low catalytic efficiency with H2O2 Taenia crassiceps ?
-
 ?
thioredoxin disulfide + NADPH + H+
-
 Taenia crassiceps thioredoxin + NADP+
-
 ?

Subunits

Subunits Comment Organism
? x * 65000, SDS-PAGE Taenia crassiceps

Synonyms

Synonyms Comment Organism
TGR
-
 Taenia crassiceps
thioredoxin-glutathione reductase
-
 Taenia crassiceps

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
 for 5,5'-dithiobis(2-nitrobenzoic acid) reductase activity Taenia crassiceps
40
-
 for glutathione reductase activity Taenia crassiceps

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.3
-
 5,5'-dithiobis(2-nitrobenzoic acid) at pH 7.8 and 25°C Taenia crassiceps
0.47
-
 thioredoxin disulfide at pH 7.8 and 25°C Taenia crassiceps
0.84
-
 GSSG at pH 7.8 and 25°C Taenia crassiceps

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 for 5,5'-dithiobis(2-nitrobenzoic acid) reductase activity Taenia crassiceps
7.8
-
 for glutathione reductase activity Taenia crassiceps

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Taenia crassiceps
NADPH
-
 Taenia crassiceps

General Information

General Information Comment Organism
physiological function the enzyme is able to protect glutamine synthetase from oxidative inactivation, suggesting that the enzyme is competent to contend with oxidative stress Taenia crassiceps

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
8.7
-
 5,5'-dithiobis(2-nitrobenzoic acid) at pH 7.8 and 25°C Taenia crassiceps
22
-
 GSSG at pH 7.8 and 25°C Taenia crassiceps
540
-
 thioredoxin disulfide at pH 7.8 and 25°C Taenia crassiceps