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Literature summary for 1.8.1.B1 extracted from

  • Song, L.; Li, J.; Xie, S.; Qian, C.; Wang, J.; Zhang, W.; Yin, X.; Hua, Z.; Yu, C.
    Thioredoxin glutathione reductase as a novel drug target: evidence from Schistosoma japonicum (2012), PLoS ONE, 7, e31456.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.8.1.B1

Application

Application Comment Organism
medicine the enzyme is a potential target for development of novel agents against schistosomes Schistosoma japonicum

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) cells Schistosoma japonicum

Inhibitors

Inhibitors Comment Organism Structure
auranofin auranofin at 0.005 mg/ml (0.0074 mM) reduces 57.1% of the thioredoxin reductase activity and 71% of the glutathione reductase activity in the Schistosoma japonicum adult worms Schistosoma japonicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00324
-
 thioredoxin disulfide at 25°C in 0.1 M potassium phosphate (pH 7.4) Schistosoma japonicum
0.01097
-
 NADPH with glutaredoxin as cosubstrate, at 25°C in 0.1 M potassium phosphate (pH 7.4) Schistosoma japonicum
0.02142
-
 NADPH with 5,5'-dithiobis(2-nitrobenzoic acid) as cosubstrate, at 25°C in 0.1 M potassium phosphate (pH 7.4) Schistosoma japonicum
0.04955
-
 GSSG at 25°C in 0.1 M potassium phosphate (pH 7.4) Schistosoma japonicum
0.08324
-
 NADPH with GSSG as cosubstrate, at 25°C in 0.1 M potassium phosphate (pH 7.4) Schistosoma japonicum
0.1451
-
 5,5'-dithiobis(2-nitrobenzoic acid) at 25°C in 0.1 M potassium phosphate (pH 7.4) Schistosoma japonicum
1.698
-
 GSH at 25°C in 0.1 M potassium phosphate (pH 7.4) Schistosoma japonicum
2.792
-
 2-hydroxyethyl disulfide at 25°C in 0.1 M potassium phosphate (pH 7.4) Schistosoma japonicum

Metals/Ions

Metals/Ions Comment Organism Structure
additional information selenoprotein Schistosoma japonicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64900
-
 x * 64900, calculated from amino acid sequence Schistosoma japonicum
65000
-
 x * 65000, SDS-PAGE Schistosoma japonicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 GSH + NADP+ Schistosoma japonicum
-
 GSSG + NADPH + H+
-
 ?
GSSG + NADPH + H+ Schistosoma japonicum
-
 GSH + NADP+
-
 ?
thioredoxin disulfide + NADPH + H+ Schistosoma japonicum
-
 thioredoxin + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Schistosoma japonicum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ADP-agarose column chromatography Schistosoma japonicum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.19
-
 with GSSG as substrate, at pH 7.4 and 25°C Schistosoma japonicum
3.33
-
 with thioredoxin disulfide as substrate, at pH 7.4 and 25°C Schistosoma japonicum
8.11
-
 with 5,5'-dithiobis(2-nitrobenzoic acid) as substrate, at pH 7.4 and 25°C Schistosoma japonicum
12.1
-
 with HED as substrate, at pH 7.4 and 25°C Schistosoma japonicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 GSH + NADP+
-
 Schistosoma japonicum GSSG + NADPH + H+
-
 ?
2-hydroxyethyl disulfide + NADPH + H+
-
 Schistosoma japonicum ?
-
 ?
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+
-
 Schistosoma japonicum 2-nitro-5-thiobenzoate + NADP+
-
 ?
glutaredoxin + NADPH + H+
-
 Schistosoma japonicum ?
-
 ?
GSSG + NADPH + H+
-
 Schistosoma japonicum GSH + NADP+
-
 ?
thioredoxin disulfide + NADPH + H+
-
 Schistosoma japonicum thioredoxin + NADP+
-
 ?

Subunits

Subunits Comment Organism
? x * 65000, SDS-PAGE Schistosoma japonicum
? x * 64900, calculated from amino acid sequence Schistosoma japonicum

Synonyms

Synonyms Comment Organism
TGR
-
 Schistosoma japonicum
thioredoxin-glutathione reductase
-
 Schistosoma japonicum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.78
-
 thioredoxin disulfide at 25°C in 0.1 M potassium phosphate (pH 7.4) Schistosoma japonicum
4.87
-
 NADPH with GSSG as cosubstrate, at 25°C in 0.1 M potassium phosphate (pH 7.4) Schistosoma japonicum
5.35
-
 GSSG at 25°C in 0.1 M potassium phosphate (pH 7.4) Schistosoma japonicum
8.16
-
 5,5'-dithiobis(2-nitrobenzoic acid) at 25°C in 0.1 M potassium phosphate (pH 7.4) Schistosoma japonicum
8.65
-
 2-hydroxyethyl disulfide at 25°C in 0.1 M potassium phosphate (pH 7.4) Schistosoma japonicum
10.92
-
 NADPH with 5,5'-dithiobis(2-nitrobenzoic acid) as cosubstrate, at 25°C in 0.1 M potassium phosphate (pH 7.4) Schistosoma japonicum
14.93
-
 NADPH with glutaredoxin as cosubstrate, at 25°C in 0.1 M potassium phosphate (pH 7.4) Schistosoma japonicum
15.78
-
 GSH at 25°C in 0.1 M potassium phosphate (pH 7.4) Schistosoma japonicum

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Schistosoma japonicum

pI Value

Organism Comment pI Value Maximum pI Value
Schistosoma japonicum calculated from amino acid sequence
-
 6.4

General Information

General Information Comment Organism
physiological function the enzyme is essential for maintaining the thiol-disulfide redox homeostasis of Schistosoma japonicum Schistosoma japonicum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1
-
 2-hydroxyethyl disulfide at 25°C in 0.1 M potassium phosphate (pH 7.4) Schistosoma japonicum
9.3
-
 GSH at 25°C in 0.1 M potassium phosphate (pH 7.4) Schistosoma japonicum
58.5
-
 NADPH with GSSG as cosubstrate, at 25°C in 0.1 M potassium phosphate (pH 7.4) Schistosoma japonicum
108
-
 GSSG at 25°C in 0.1 M potassium phosphate (pH 7.4) Schistosoma japonicum
119
-
 5,5'-dithiobis(2-nitrobenzoic acid) at 25°C in 0.1 M potassium phosphate (pH 7.4) Schistosoma japonicum
510
-
 NADPH with 5,5'-dithiobis(2-nitrobenzoic acid) as cosubstrate, at 25°C in 0.1 M potassium phosphate (pH 7.4) Schistosoma japonicum
1360
-
 NADPH with glutaredoxin as cosubstrate, at 25°C in 0.1 M potassium phosphate (pH 7.4) Schistosoma japonicum
1480
-
 thioredoxin disulfide at 25°C in 0.1 M potassium phosphate (pH 7.4) Schistosoma japonicum