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Literature summary for 1.8.2.1 extracted from

  • Charles, A.M.; Suzuki, I.
    Purification and properties of sulfite:cytochrome c oxidoreductase from Thiobacillus novellus (1966), Biochim. Biophys. Acta, 128, 522-534.
No PubMed abstract available

General Stability

General Stability Organism
stable to repeated freezing and thawing Starkeya novella

Inhibitors

Inhibitors Comment Organism Structure
AMP 0.01 mM, 25% inhibition, activity with ferricyanide Starkeya novella
EDTA 0.005 mM, 20% inhibition Starkeya novella
HgCl2 0.667 mM, 56% inhibition Starkeya novella
additional information CaCl2, MgSO4, ZnSO4, CuSO4, FeCl3, NiCl2 and Na2MoO4 inhibits the reaction by 70% at 0.267 mM, this effect is apparently due to the non-enzymatic oxidation of sulfite with air Starkeya novella
NaAsO2 31% inhibition Starkeya novella
NaCl competitive with respect to sulfite Starkeya novella
NaF 0.01 mM, 17.5% inhibition Starkeya novella
NEM 0.667 mM, 30% loss of activity Starkeya novella
p-hydroxymercuribenzoate 0.667 mM, 86% loss of activity, inhibition is completely reversed by GSH Starkeya novella

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
sulfite pH 6.5, reaction with cytochrome c Starkeya novella
0.02
-
ferricyanide pH 8.0 Starkeya novella
0.04
-
sulfite pH 8.0, reaction with cytochrome c Starkeya novella

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sulfite + ferricytochrome c + H2O Starkeya novella oxidative phosphorylation is coupled to sulfite oxidation with a low P/O ratio sulfate + ferrocytochrome c + H+
-
?

Organic Solvent Stability

Organic Solvent Comment Organism
Ethanol 50%, significant loss of activity Starkeya novella

Organism

Organism UniProt Comment Textmining
Starkeya novella
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Starkeya novella

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
139.5
-
reaction with ferricyanide Starkeya novella

Storage Stability

Storage Stability Organism
-20°C, retains high activity for more than 6 months Starkeya novella

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sulfite + ferricyanide + H2O
-
Starkeya novella sulfate + ferrocyanide + H+
-
?
sulfite + ferricytochrome c + H2O specific for sulfite Starkeya novella sulfate + ferrocytochrome c + H+
-
?
sulfite + ferricytochrome c + H2O oxidative phosphorylation is coupled to sulfite oxidation with a low P/O ratio Starkeya novella sulfate + ferrocytochrome c + H+
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
complete loss of activity after 5 min, 25% loss of activity after 5 min in presence of 0.05 M sodium sulfite Starkeya novella
60
-
1 min, complete loss of activity Starkeya novella

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
potassium phosphate buffer Starkeya novella

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.5
-
NaCl
-
Starkeya novella