Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ni2+ | 1 mM, 32% inhibition | Paracoccus pantotrophus | |
phosphate | - |
Paracoccus pantotrophus | |
SO42- | - |
Paracoccus pantotrophus | |
Zn2+ | 1 mM, 27% inhibition | Paracoccus pantotrophus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
sulfite | - |
Paracoccus pantotrophus | |
0.019 | - |
ferricytochrome c | - |
Paracoccus pantotrophus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Paracoccus pantotrophus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | enzyme contains 3.17 mol of iron per mol of enzyme | Paracoccus pantotrophus | |
Mg2+ | 1 mM, 150% increase in activity | Paracoccus pantotrophus | |
Mn2+ | 1 mM, 127% increase in activity | Paracoccus pantotrophus | |
Molybdenum | the 43897 Da subunit contains the 455 Da molybdenum cofactor, 1.3 mol of molybdenum per mol of enzyme | Paracoccus pantotrophus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38815 | - |
2 * 43897 + 2 * 38815, the 43897 Da subunit contains the 455 Da molybdenum cofactor, the 38815 Da subunit consists of a polypeptide and two covalently bound hemes, electrospray ionization mass spectrometry | Paracoccus pantotrophus |
43897 | - |
2 * 43897 + 2 * 38815, the 43897 Da subunit contains the 455 Da molybdenum cofactor, the 38815 Da subunit consists of a polypeptide and two covalently bound hemes, electrospray ionization mass spectrometry | Paracoccus pantotrophus |
47000 | - |
alpha2beta2, 2 * 47000 + 2 * 50000, SDS-PAGE | Paracoccus pantotrophus |
50000 | - |
alpha2beta2, 2 * 47000 + 2 * 50000, SDS-PAGE | Paracoccus pantotrophus |
190000 | - |
native gradient PAGE | Paracoccus pantotrophus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paracoccus pantotrophus | - |
GB17 | - |
Paracoccus pantotrophus GB17 | - |
GB17 | - |
Purification (Comment) | Organism |
---|---|
- |
Paracoccus pantotrophus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.049 | - |
- |
Paracoccus pantotrophus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sulfite + ferricytochrome c + H2O | - |
Paracoccus pantotrophus | sulfate + ferrocytochrome c + H+ | - |
? | |
sulfite + ferricytochrome c + H2O | - |
Paracoccus pantotrophus GB17 | sulfate + ferrocytochrome c + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 2 * 43897 + 2 * 38815, the 43897 Da subunit contains the 455 Da molybdenum cofactor, the 38815 Da subunit consists of a polypeptide and two covalently bound hemes, electrospray ionization mass spectrometry | Paracoccus pantotrophus |
tetramer | alpha2beta2, 2 * 47000 + 2 * 50000, SDS-PAGE | Paracoccus pantotrophus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Paracoccus pantotrophus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | the 38815 Da subunit consists of a polypeptide and two covalently bound hemes, 3.53 mol of heme per mol of enzyme | Paracoccus pantotrophus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
45 | - |
phosphate | - |
Paracoccus pantotrophus |