Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.8.2.1 extracted from

  • Quentmeier, A.; Kraft, R.; Kostka, S.; Klockenkamper, R.; Friedrich, C.G.
    Characterization of a new type of sulfite dehydrogenase from Paracoccus pantotrophus GB17 (2000), Arch. Microbiol., 173, 117-125.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Ni2+ 1 mM, 32% inhibition Paracoccus pantotrophus
phosphate
-
Paracoccus pantotrophus
SO42-
-
Paracoccus pantotrophus
Zn2+ 1 mM, 27% inhibition Paracoccus pantotrophus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
sulfite
-
Paracoccus pantotrophus
0.019
-
ferricytochrome c
-
Paracoccus pantotrophus

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
Paracoccus pantotrophus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Iron enzyme contains 3.17 mol of iron per mol of enzyme Paracoccus pantotrophus
Mg2+ 1 mM, 150% increase in activity Paracoccus pantotrophus
Mn2+ 1 mM, 127% increase in activity Paracoccus pantotrophus
Molybdenum the 43897 Da subunit contains the 455 Da molybdenum cofactor, 1.3 mol of molybdenum per mol of enzyme Paracoccus pantotrophus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38815
-
2 * 43897 + 2 * 38815, the 43897 Da subunit contains the 455 Da molybdenum cofactor, the 38815 Da subunit consists of a polypeptide and two covalently bound hemes, electrospray ionization mass spectrometry Paracoccus pantotrophus
43897
-
2 * 43897 + 2 * 38815, the 43897 Da subunit contains the 455 Da molybdenum cofactor, the 38815 Da subunit consists of a polypeptide and two covalently bound hemes, electrospray ionization mass spectrometry Paracoccus pantotrophus
47000
-
alpha2beta2, 2 * 47000 + 2 * 50000, SDS-PAGE Paracoccus pantotrophus
50000
-
alpha2beta2, 2 * 47000 + 2 * 50000, SDS-PAGE Paracoccus pantotrophus
190000
-
native gradient PAGE Paracoccus pantotrophus

Organism

Organism UniProt Comment Textmining
Paracoccus pantotrophus
-
GB17
-
Paracoccus pantotrophus GB17
-
GB17
-

Purification (Commentary)

Purification (Comment) Organism
-
Paracoccus pantotrophus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.049
-
-
Paracoccus pantotrophus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sulfite + ferricytochrome c + H2O
-
Paracoccus pantotrophus sulfate + ferrocytochrome c + H+
-
?
sulfite + ferricytochrome c + H2O
-
Paracoccus pantotrophus GB17 sulfate + ferrocytochrome c + H+
-
?

Subunits

Subunits Comment Organism
tetramer 2 * 43897 + 2 * 38815, the 43897 Da subunit contains the 455 Da molybdenum cofactor, the 38815 Da subunit consists of a polypeptide and two covalently bound hemes, electrospray ionization mass spectrometry Paracoccus pantotrophus
tetramer alpha2beta2, 2 * 47000 + 2 * 50000, SDS-PAGE Paracoccus pantotrophus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Paracoccus pantotrophus

Cofactor

Cofactor Comment Organism Structure
heme the 38815 Da subunit consists of a polypeptide and two covalently bound hemes, 3.53 mol of heme per mol of enzyme Paracoccus pantotrophus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
45
-
phosphate
-
Paracoccus pantotrophus