Inhibitors | Comment | Organism | Structure |
---|---|---|---|
azide | 63% inhibition at 0.5 mM | Acidiphilium acidophilum | |
EDTA | not inhibitory | Acidiphilium acidophilum | |
N-ethylmaleimide | not inhibitory | Acidiphilium acidophilum | |
Na2SeO3 | 39% inhibition at 0.5 mM | Acidiphilium acidophilum | |
Na2SeO4 | 51% inhibition at 5 mM | Acidiphilium acidophilum | |
p-chloromercuribenzoate | 32% inhibition at 0.1 mM | Acidiphilium acidophilum | |
SO32- | 100% inhibition at 0.5 mM | Acidiphilium acidophilum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.54 | - |
thiosulfate | at pH 7 | Acidiphilium acidophilum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
2 * 24000 + 2 * 20000, SDS-PAGE, both subunits contain c553-type heme | Acidiphilium acidophilum |
24000 | - |
2 * 24000 + 2 * 20000, SDS-PAGE, both subunits contain c553-type heme | Acidiphilium acidophilum |
102000 | - |
gel filtration | Acidiphilium acidophilum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidiphilium acidophilum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using ammonium sulfate precipitation, hydrophobic interaction chromatography, anion-exchange chromatography and gel filtration | Acidiphilium acidophilum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4925 | - |
purified enzyme | Acidiphilium acidophilum |
Storage Stability | Organism |
---|---|
frozen, stable for at least 3 months | Acidiphilium acidophilum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity with: pyrrolo-quinoline quinone, methyl- or benzyl viologen, methylthiazol tetrazolium bromide | Acidiphilium acidophilum | ? | - |
? | |
additional information | NAD+, methylene blue, 2,6-dichlorophenol indophenol | Acidiphilium acidophilum | ? | - |
? | |
additional information | no activity with: NADP+, O2 | Acidiphilium acidophilum | ? | - |
? | |
thiosulfate + oxidized acceptor | phenazine methosulfate and phenazine ethosulfate assayed in the presence of dichlorophenolindophenol and Thialhalivibrio versutus cytochrome c550 support low, but significant enzyme activities | Acidiphilium acidophilum | tetrathionate + reduced acceptor | - |
? | |
thiosulfate + oxidized acceptor | mammalian cytochrome c acts as electron acceptor | Acidiphilium acidophilum | tetrathionate + reduced acceptor | - |
? | |
thiosulfate + oxidized acceptor | Wurster's blue can also serve as electron acceptor | Acidiphilium acidophilum | tetrathionate + reduced acceptor | - |
? | |
thiosulfate + oxidized acceptor | ferricyanide is a good electron acceptor | Acidiphilium acidophilum | tetrathionate + reduced acceptor | - |
? | |
thiosulfate + oxidized acceptor | ferricyanide acts as electron acceptor | Acidiphilium acidophilum | tetrathionate + reduced acceptor | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | 2 * 24000 + 2 * 20000, SDS-PAGE, both subunits contain c553-type heme | Acidiphilium acidophilum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
- |
20% decrease of activity in 2 h and 60% decrease of activity in 7 h | Acidiphilium acidophilum |
30 | - |
50% decrease of activity in 2 h | Acidiphilium acidophilum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8500 | - |
thiosulfate | at pH 7 | Acidiphilium acidophilum | |
250000 | - |
thiosulfate | at least, at pH 3 | Acidiphilium acidophilum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | - |
purified enzyme | Acidiphilium acidophilum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2 | - |
unstable below | Acidiphilium acidophilum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme c | 5.3 mole per mol of native enzyme, made up of two different c-553 hemes, present in both subunit types | Acidiphilium acidophilum |