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Literature summary for 1.8.2.2 extracted from

  • Visser, J.M.; De Jong, G.A.H.; Robertson, L.A.; Kuenen, J.G.
    Purification and characterization of a periplasmic thiosulfate dehydrogenase from the obligately autotrophic Thiobacillus sp. W5 (1996), Arch. Microbiol., 166, 372-378.
    View publication on PubMed
Search for more literature for 1.8.2.2

Inhibitors

Inhibitors Comment Organism Structure
cyanide not inhibitory Thiobacillus sp.
EDTA not inhibitory Thiobacillus sp.
N-ethylmaleimide not inhibitory Thiobacillus sp.
Na2SeO3 it inhibits the oxidation of thiosulfate by whole cells Thiobacillus sp.
SO32- inhibits at concentrations above 0.05 mM, the sulfite inhibition of thiosulfate oxidation by whole cells is very similar to the inhibition pattern of the enzyme Thiobacillus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
 thiosulfate at pH 7.5 Thiobacillus sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm at least 73% of the enzyme activity is recovered in the periplasmic fraction Thiobacillus sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
 2 * 33000 + 2 * 27000, SDS-PAGE Thiobacillus sp.
33000
-
 2 * 33000 + 2 * 27000, SDS-PAGE Thiobacillus sp.
120000
-
 gel filtration Thiobacillus sp.

Organism

Organism UniProt Comment Textmining
Thiobacillus sp.
-
 W5
-
Thiobacillus sp. W5
-
 W5
-

Purification (Commentary)

Purification (Comment) Organism
using chromatography on Phenyl-Sepharose column and Mono Q column, gel filtration on Superdex 200 and column chromatography on Phenyl Superose Thiobacillus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1149
-
 purified enzyme Thiobacillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with: benzyl viologen, 2,6-dichloroindophenol, 2,6-dichloroindophenol with phenazine methosulfate, 2,6-dichloroindophenol with phenazine ethosulfate, horse heart cytochrome c with phenazine methosulfate, horse heart cytochrome c with phenazine ethosulfate Thiobacillus sp. ?
-
 ?
additional information NAD+, methylene blue, 2,6-dichlorophenol indophenol Thiobacillus sp. ?
-
 ?
additional information no activity with: benzyl viologen, 2,6-dichloroindophenol, 2,6-dichloroindophenol with phenazine methosulfate, 2,6-dichloroindophenol with phenazine ethosulfate, horse heart cytochrome c with phenazine methosulfate, horse heart cytochrome c with phenazine ethosulfate Thiobacillus sp. W5 ?
-
 ?
additional information NAD+, methylene blue, 2,6-dichlorophenol indophenol Thiobacillus sp. W5 ?
-
 ?
thiosulfate + oxidized acceptor strictly specific for thiosulfate Thiobacillus sp. tetrathionate + reduced acceptor
-
 ?
thiosulfate + oxidized acceptor the activity does not couple with mammalian cytochrome c as electron acceptor Thiobacillus sp. tetrathionate + reduced acceptor
-
 ?
thiosulfate + oxidized acceptor ferricyanide acts as electron acceptor Thiobacillus sp. tetrathionate + reduced acceptor
-
 ?
thiosulfate + oxidized acceptor strictly specific for thiosulfate Thiobacillus sp. W5 tetrathionate + reduced acceptor
-
 ?
thiosulfate + oxidized acceptor the activity does not couple with mammalian cytochrome c as electron acceptor Thiobacillus sp. W5 tetrathionate + reduced acceptor
-
 ?
thiosulfate + oxidized acceptor ferricyanide acts as electron acceptor Thiobacillus sp. W5 tetrathionate + reduced acceptor
-
 ?

Subunits

Subunits Comment Organism
heterotetramer 2 * 33000 + 2 * 27000, SDS-PAGE Thiobacillus sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2300
-
 thiosulfate
-
 Thiobacillus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Thiobacillus sp.

Cofactor

Cofactor Comment Organism Structure
heme c 4 mol per mol of enzyme, present in both subunit types Thiobacillus sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.03
-
 Na2SeO3 it is possible that selenite blocks access to the thiosulfate dehydrogenase or has an inhibiting effect on the respiratory chain itself Thiobacillus sp.
0.3
-
 SO32- sulfite resembles the sulfonate part of thiosulfate and might, therefore, block the active site of the enzyme Thiobacillus sp.