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Literature summary for 1.8.2.3 extracted from

  • Quentmeier, A.; Hellwig, P.; Bardischewsky, F.; Wichmann, R.; Friedrich, C.
    Sulfide dehydrogenase activity of the monomeric flavoprotein SoxF of Paracoccus pantotrophus (2004), Biochemistry, 43, 14696-14703.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
cyanide 1 mM cyanide inhibits SoxF activity at pH 6.0 by 25% and at pH 8.0 by 92% Paracoccus pantotrophus
reduced heart cytochrome c competitive product inhibition Paracoccus pantotrophus
sulfite preincubation of SoxF with 1 mM sodium sulfite for 20 min inactivates SoxF (9.0% residual sulfide dehydrogenase activity) Paracoccus pantotrophus
sulfur
-
Paracoccus pantotrophus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0023
-
Sulfide at pH 6.0 and 30°C Paracoccus pantotrophus
0.116
-
oxidized heart cytochrome c at pH 6.0 and 30°C Paracoccus pantotrophus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42010
-
calculated from amino acid sequence Paracoccus pantotrophus
42797
-
1 * 42797, electrospray mass spectrometry Paracoccus pantotrophus
42832
-
1 * 42832, electrospray mass spectrometry of SoxF as isolated yields masses of 42797 Da and 42832 Da Paracoccus pantotrophus

Organism

Organism UniProt Comment Textmining
Paracoccus pantotrophus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and Q-Sepharose column chromatography Paracoccus pantotrophus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information SoxF does not catalyze thiosulfate- or sulfite-dependent cytochrome c reduction Paracoccus pantotrophus ?
-
?
sulfide + oxidized heart cytochrome c
-
Paracoccus pantotrophus sulfur + reduced heart cytochrome c + H+ sulfur or polysulfide as product ?

Subunits

Subunits Comment Organism
monomer 1 * 42797, electrospray mass spectrometry Paracoccus pantotrophus
monomer 1 * 42832, electrospray mass spectrometry of SoxF as isolated yields masses of 42797 Da and 42832 Da Paracoccus pantotrophus

Synonyms

Synonyms Comment Organism
FCSD
-
Paracoccus pantotrophus
flavocytochrome c-sulfide dehydrogenase
-
Paracoccus pantotrophus
SoxF
-
Paracoccus pantotrophus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.9
-
oxidized heart cytochrome c at pH 6.0 and 30°C Paracoccus pantotrophus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Paracoccus pantotrophus

Cofactor

Cofactor Comment Organism Structure
FAD
-
Paracoccus pantotrophus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0013
-
sulfur at pH 6.0 and 30°C Paracoccus pantotrophus
0.0072
-
reduced heart cytochrome c at pH 6.0 and 30°C Paracoccus pantotrophus