Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cyanide | 1 mM cyanide inhibits SoxF activity at pH 6.0 by 25% and at pH 8.0 by 92% | Paracoccus pantotrophus | |
reduced heart cytochrome c | competitive product inhibition | Paracoccus pantotrophus | |
sulfite | preincubation of SoxF with 1 mM sodium sulfite for 20 min inactivates SoxF (9.0% residual sulfide dehydrogenase activity) | Paracoccus pantotrophus | |
sulfur | - |
Paracoccus pantotrophus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0023 | - |
Sulfide | at pH 6.0 and 30°C | Paracoccus pantotrophus | |
0.116 | - |
oxidized heart cytochrome c | at pH 6.0 and 30°C | Paracoccus pantotrophus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42010 | - |
calculated from amino acid sequence | Paracoccus pantotrophus |
42797 | - |
1 * 42797, electrospray mass spectrometry | Paracoccus pantotrophus |
42832 | - |
1 * 42832, electrospray mass spectrometry of SoxF as isolated yields masses of 42797 Da and 42832 Da | Paracoccus pantotrophus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paracoccus pantotrophus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and Q-Sepharose column chromatography | Paracoccus pantotrophus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | SoxF does not catalyze thiosulfate- or sulfite-dependent cytochrome c reduction | Paracoccus pantotrophus | ? | - |
? | |
sulfide + oxidized heart cytochrome c | - |
Paracoccus pantotrophus | sulfur + reduced heart cytochrome c + H+ | sulfur or polysulfide as product | ? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 42797, electrospray mass spectrometry | Paracoccus pantotrophus |
monomer | 1 * 42832, electrospray mass spectrometry of SoxF as isolated yields masses of 42797 Da and 42832 Da | Paracoccus pantotrophus |
Synonyms | Comment | Organism |
---|---|---|
FCSD | - |
Paracoccus pantotrophus |
flavocytochrome c-sulfide dehydrogenase | - |
Paracoccus pantotrophus |
SoxF | - |
Paracoccus pantotrophus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.9 | - |
oxidized heart cytochrome c | at pH 6.0 and 30°C | Paracoccus pantotrophus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Paracoccus pantotrophus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Paracoccus pantotrophus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0013 | - |
sulfur | at pH 6.0 and 30°C | Paracoccus pantotrophus | |
0.0072 | - |
reduced heart cytochrome c | at pH 6.0 and 30°C | Paracoccus pantotrophus |