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Literature summary for 1.8.3.1 extracted from

  • D'Errico, G.; Di Salle, A.; La Cara, F.; Rossi, M.; Cannio, R.
    Identification and characterization of a novel bacterial sulfite oxidase with no heme binding domain from Deinococcus radiodurans (2006), J. Bacteriol., 188, 694-701.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.8.3.1

Activating Compound

Activating Compound Comment Organism Structure
Tris-acetate up to a concentration of 70 mM, up to 2fold activation Deinococcus radiodurans
Tris-HCl up to a concentration of 70 mM, up to 2fold activation Deinococcus radiodurans

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strains BL21-CodonPlus(DE3)-RIL and TP1000 Deinococcus radiodurans

Inhibitors

Inhibitors Comment Organism Structure
EDTA 20 mM, 50% inhibition Deinococcus radiodurans
imidazole 0.1 mM, complete inhibition Deinococcus radiodurans
KCl 30 mM, 50% inhibition Deinococcus radiodurans
KNO3 3 mM, 50% inhibition Deinococcus radiodurans
NaCl 150 mM, 50% inhibition Deinococcus radiodurans
NiCl2 0.1 mM, complete inhibition Deinococcus radiodurans
potassium phosphate 20 mM, pH 8.0, 50% inhibition Deinococcus radiodurans
Sodium arsenate 7 mM, 50% inhibition Deinococcus radiodurans
Sodium sulfate 20 mM, 50% inhibition Deinococcus radiodurans
Tris-acetate 80 mM, pH 8.0, 50% inhibition Deinococcus radiodurans
Tris-HCl 100 mM, pH 8.0, 50% inhibition Deinococcus radiodurans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0945
-
 sulfite
-
 Deinococcus radiodurans

Localization

Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl up to a concentration of 100 mM, up to 2fold activation Deinococcus radiodurans
Sodium arsenate up to a concentration of 5 mM, up to 2fold activation Deinococcus radiodurans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38940
-
 calculated from sequence of cDNA Deinococcus radiodurans
40250
-
 2 * 40250, gel filtration Deinococcus radiodurans
43000
-
 His-tagged enzyme, SDS-PAGE Deinococcus radiodurans
44300
-
 gel filtration Deinococcus radiodurans

Organism

Organism UniProt Comment Textmining
Deinococcus radiodurans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His-Select HF nickel affinity gel column chromatography Deinococcus radiodurans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sulfite + H2O + A
-
 Deinococcus radiodurans SO42- + AH2
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 40250, gel filtration Deinococcus radiodurans

Synonyms

Synonyms Comment Organism
SO
-
 Deinococcus radiodurans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Deinococcus radiodurans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 60
-
 Deinococcus radiodurans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45 60 half-lives of 10 min at 60°C, 30 min at 55°C, and 3 h at 45°C, respectively Deinococcus radiodurans

pI Value

Organism Comment pI Value Maximum pI Value
Deinococcus radiodurans
-
-
 6.09