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Literature summary for 1.8.3.1 extracted from

  • Spricigo, R.; Dronov, R.; Rajagopalan, K.V.; Lisdat, F.; Leimkuehler, S.; Scheller, F.W.; Wollenberger, U.
    Electrocatalytically functional multilayer assembly of sulfite oxidase and cytochrome c (2008), Soft Matter, 4, 972-978.
    View publication on PubMed

Application

Application Comment Organism
food industry artificial ETC composed of cytochrome c and sulfite oxidase formed by the layer-by-layer technique using a polyelectrolyte. The multilayer technology, e.g. sulfite oxidase-cyt c multilayer electrode may act as an anode in a bio-fuel cell and furthermore such multilayers may be exploited as a biosensor for the detection of sulfite, which is used as a preservative in wine and other foodstuffs Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged SO expressed in Escherichia coli TP1000 cells containing plasmid pTG718 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
sulfite
-
Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
on Ni-NTA resin Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sulfite + cytochrome c
-
Homo sapiens sulfate + reduced cytochrome c
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Homo sapiens

pI Value

Organism Comment pI Value Maximum pI Value
Homo sapiens
-
-
5.5