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Literature summary for 1.8.3.2 extracted from

  • Hoober, K.L.; Joneja, B.; White, H.B.; Thorpe, C.
    A sulfhydryl oxidase from chicken egg white (1996), J. Biol. Chem., 271, 30510-30516.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
iodoacetamide
-
Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
dithiothreitol
-
Gallus gallus
10.9
-
L-Cys
-
Gallus gallus
20
-
GSH
-
Gallus gallus
54
-
2-mercaptoethanol
-
Gallus gallus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
93000
-
2 * 93000, SDS-PAGE Gallus gallus
202000
-
gel filtration Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Gallus gallus

Purification (Commentary)

Purification (Comment) Organism
-
Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
egg egg white Gallus gallus
-

Storage Stability

Storage Stability Organism
-20°C, stable for months Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-mercaptoethanol + O2
-
Gallus gallus ? + H2O
-
?
dithiothreitol + O2 anaerobically, the ferricenium ion is a facile alternative electron acceptor Gallus gallus ? + H2O production of H2O2 ?
GSH + O2 + O2
-
Gallus gallus GSSG + H2O
-
?
L-Cys + O2
-
Gallus gallus ? + H2O
-
?

Subunits

Subunits Comment Organism
dimer 2 * 93000, SDS-PAGE Gallus gallus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
17.2
-
dithiothreitol
-
Gallus gallus
20.3
-
2-mercaptoethanol
-
Gallus gallus
21.3
-
L-Cys
-
Gallus gallus
23.1
-
GSH
-
Gallus gallus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Gallus gallus

pH Range

pH Minimum pH Maximum Comment Organism
5 11 pH 5.0: about 40% of maximal activity, pH 11: about 70% of maximal activity Gallus gallus

Cofactor

Cofactor Comment Organism Structure
FAD
-
Gallus gallus