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Literature summary for 1.8.3.3 extracted from

  • Compagnone, D.; Federici, G.; Scarciglia, L.; Palleschi, G.
    Amperometric glutathione electrodes (1993), Biosens. Bioelectron., 8, 257-263.
No PubMed abstract available
Search for more literature for 1.8.3.3

Application

Application Comment Organism
analysis development of amperometric glutathione electrodes with immobilized enzyme for detection of H2O2 Penicillium sp.

General Stability

General Stability Organism
after 50 measurements with enzyme immobilized on microelectrode sensor loss of 50% activity Penicillium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glutathione + O2 Penicillium sp. measurement of H2O2 in vivo via microelectrodes with immobilized enzyme glutathione disulfide + H2O2
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 ?

Organism

Organism UniProt Comment Textmining
Penicillium sp.
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Storage Stability

Storage Stability Organism
4°C, pH 6.5, phosphate buffer, immobilized enzyme on microelectrode sensor, loss of 20% activity, 60 days Penicillium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-mercaptoethanol + O2 19% of the activity with glutathione Penicillium sp. mercaptoethanol disulfide
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 ?
D-cysteine + O2 low activity Penicillium sp. D-cystine
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 ?
dithiothreitol + O2 38% of the activity with glutathione Penicillium sp. ?
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 ?
glutathione + O2 enzyme immobilized on microsensor Penicillium sp. glutathione disulfide + H2O2
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 ?
glutathione + O2 measurement of H2O2 in vivo via microelectrodes with immobilized enzyme Penicillium sp. glutathione disulfide + H2O2
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 ?
L-cysteine + O2 low activity Penicillium sp. L-cystine
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 ?
L-cysteine ethyl ester + O2 low activity Penicillium sp. L-cystine diethyl ester
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 ?
additional information D-penicilamine and coenzyme A are no substrates Penicillium sp. ?
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 ?
N-acetyl-L-cysteine + O2 low activity Penicillium sp. N,N'-diacetyl-L-cystine
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 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay with immobilized enzyme on microsensor Penicillium sp.