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Literature summary for 1.8.3.3 extracted from
- Augmenter of liver regeneration: a flavin-dependent sulfhydryl oxidase with cytochrome c reductase activity (2005), Biochemistry, 44, 1532-1541.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C124A | reduced activity compared to wild type enzyme | Homo sapiens |
| C15A | reduced activity compared to wild type enzyme | Homo sapiens |
| C15A/C124A | reduced activity compared to wild type enzyme | Homo sapiens |
| C15A/C74A/C85A/C124A | reduced activity compared to wild type enzyme | Homo sapiens |
| C74A/C85A | reduced activity compared to wild type enzyme | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
cytochrome c | wild type enzyme | Homo sapiens |  |
| 1.3 | - |
dithiothreitol | mutant enzyme C15A | Homo sapiens | |
| 1.7 | - |
dithiothreitol | mutant enzyme C15A/C74A/C85A/C124A | Homo sapiens | |
| 1.8 | - |
dithiothreitol | mutant enzyme C124A | Homo sapiens | |
| 2 | - |
dithiothreitol | mutant enzyme C15A/C124A | Homo sapiens | |
| 2 | - |
dithiothreitol | mutant enzyme C74A/C85A | Homo sapiens | |
| 2.1 | - |
dithiothreitol | wild type enzyme | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| gel filtration | Homo sapiens |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| in 8M urea | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dithiothreitol + cytochrome c | - |
Homo sapiens | dithiothreitol disulfide + reduced cytochrome c | - |
? | |
| dithiothreitol + O2 | - |
Homo sapiens | dithiothreitol disulfide + H2O2 | - |
? | |
| R-SH + O2 | - |
Homo sapiens | R-S-S-R + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | disulfide-bridged dimer (linked via C15-C124) with two free cysteine residues (C74 and 85) per monomer | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| flavin-dependent sulfhydryl oxidase | - |
Homo sapiens |
| flavin-linked sulfhydryl oxidase | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.83 | - |
dithiothreitol | mutant enzyme C74A/C85A | Homo sapiens | |
| 0.867 | - |
dithiothreitol | mutant enzyme C15A | Homo sapiens | |
| 0.9 | - |
dithiothreitol | mutant enzyme C124A | Homo sapiens | |
| 0.95 | - |
dithiothreitol | mutant enzyme C15A/C124A | Homo sapiens | |
| 1.1 | - |
dithiothreitol | wild type enzyme | Homo sapiens | |
| 1.25 | - |
dithiothreitol | mutant enzyme C15A/C74A/C85A/C124A | Homo sapiens | |
| 4.5 | - |
cytochrome c | wild type enzyme | Homo sapiens | |
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Release 2023.1 (January 2023)
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