Cloned (Comment) | Organism |
---|---|
gene msrA | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
C52S | site-directed mutagenesis, reduced activity compared to the wild-type, no complementation of a msrA knockout mutant | Escherichia coli |
additional information | a knockout mutant shows reduced ability to attach to host lung and vein epithelial cells | Streptococcus pneumoniae |
additional information | construction of knockout mutants which show higher sensitivity to hydrogen peroxide compared to wild-type cells which can be compensated by complementation with the wild-type msrA gene from either Escherichia coli or Mycobacterium tuberculosis, but a mutant C52S msrA gene cannot restore activity in the knockout mutant strain, mutants show reduced type 1 fimbriae-mediated mannose-dependent agglutination of erythrocytes | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
calmodulin-L-methionine (S)-sulfoxide + thioredoxin | Escherichia coli | - |
calmodulin-L-methionine + thioredoxin disulfide + H2O | - |
? | |
L-methionine (S)-sulfoxide + thioredoxin | Streptococcus pneumoniae | - |
L-methionine + thioredoxin disulfide + H2O | - |
? | |
L-methionine (S)-sulfoxide + thioredoxin | Escherichia coli | substrates are several peptides and proteins, overview | L-methionine + thioredoxin disulfide + H2O | - |
? | |
additional information | Escherichia coli | the enzyme is important in protection of the cell against oxidative damage by oxidation of methionine residues in proteins, biological function | ? | - |
? | |
additional information | Streptococcus pneumoniae | the enzyme is important in protection of the cell against oxidative damage by oxidation of methionine residues in proteins, biological function | ? | - |
? | |
ribosomal protein L12-L-methionine (S)-sulfoxide + thioredoxin | Escherichia coli | - |
ribosomal protein L12-L-methionine + thioredoxin disulfide + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Streptococcus pneumoniae | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-methionine (S)-sulfoxide + thioredoxin = L-methionine + thioredoxin disulfide + H2O | reaction mechanism | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
calmodulin-L-methionine (S)-sulfoxide + thioredoxin | - |
Escherichia coli | calmodulin-L-methionine + thioredoxin disulfide + H2O | - |
? | |
L-methionine (S)-sulfoxide + 2 dithiothreitol | - |
Escherichia coli | L-methionine + dithiothreitol disulfide + H2O | - |
? | |
L-methionine (S)-sulfoxide + thioredoxin | - |
Escherichia coli | L-methionine + thioredoxin disulfide + H2O | - |
? | |
L-methionine (S)-sulfoxide + thioredoxin | - |
Streptococcus pneumoniae | L-methionine + thioredoxin disulfide + H2O | - |
? | |
L-methionine (S)-sulfoxide + thioredoxin | substrates are several peptides and proteins, overview | Escherichia coli | L-methionine + thioredoxin disulfide + H2O | - |
? | |
additional information | MsrA is specific for the S-form of the substrate | Escherichia coli | ? | - |
? | |
additional information | the enzyme is important in protection of the cell against oxidative damage by oxidation of methionine residues in proteins, biological function | Escherichia coli | ? | - |
? | |
additional information | the enzyme is important in protection of the cell against oxidative damage by oxidation of methionine residues in proteins, biological function | Streptococcus pneumoniae | ? | - |
? | |
ribosomal protein L12-L-methionine (S)-sulfoxide + thioredoxin | - |
Escherichia coli | ribosomal protein L12-L-methionine + thioredoxin disulfide + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MsrA | - |
Escherichia coli |
MsrA | - |
Streptococcus pneumoniae |
peptide methionine sulfoxide reductase | - |
Escherichia coli |
peptide methionine sulfoxide reductase | - |
Streptococcus pneumoniae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | utilized in vitro | Escherichia coli | |
thioredoxin | physiologic cofactor | Escherichia coli |