Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dimethyl sulfoxide | DMSO competitively inhibits the methionine-sulfoxide reduction ability of MsrA (12% residual activity at 0.1% (v/v) DMSO) and inhibits the antioxidant function of MsrA in yeast cells, resulting in higher sensitivity to oxidative stress | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-methionine (S)-sulfoxide + 2 dithiothreitol | Saccharomyces cerevisiae | - |
L-methionine + dithiothreitol disulfide + H2O | - |
? | |
L-methionine (S)-sulfoxide + 2 dithiothreitol | Saccharomyces cerevisiae BY4741 | - |
L-methionine + dithiothreitol disulfide + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae BY4741 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Talon-metal affinity column chromatography | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dabsyl-L-methionine-(S)-S-sulfoxide + dithiothreitol | - |
Saccharomyces cerevisiae | dabsyl-L-methionine + dithiothreitol disulfide + H2O | - |
? | |
dabsyl-L-methionine-(S)-S-sulfoxide + dithiothreitol | - |
Saccharomyces cerevisiae BY4741 | dabsyl-L-methionine + dithiothreitol disulfide + H2O | - |
? | |
L-methionine (S)-sulfoxide + 2 dithiothreitol | - |
Saccharomyces cerevisiae | L-methionine + dithiothreitol disulfide + H2O | - |
? | |
L-methionine (S)-sulfoxide + 2 dithiothreitol | - |
Saccharomyces cerevisiae BY4741 | L-methionine + dithiothreitol disulfide + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
methionine sulfoxide reductase A | - |
Saccharomyces cerevisiae |
MsrA | - |
Saccharomyces cerevisiae |