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Literature summary for 1.8.4.11 extracted from

  • Kwak, G.H.; Choi, S.H.; Kim, H.Y.
    Dimethyl sulfoxide elevates hydrogen peroxide-mediated cell death in Saccharomyces cerevisiae by inhibiting the antioxidant function of methionine sulfoxide reductase A (2010), BMB Rep., 43, 622-628.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
dimethyl sulfoxide DMSO competitively inhibits the methionine-sulfoxide reduction ability of MsrA (12% residual activity at 0.1% (v/v) DMSO) and inhibits the antioxidant function of MsrA in yeast cells, resulting in higher sensitivity to oxidative stress Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-methionine (S)-sulfoxide + 2 dithiothreitol Saccharomyces cerevisiae
-
L-methionine + dithiothreitol disulfide + H2O
-
?
L-methionine (S)-sulfoxide + 2 dithiothreitol Saccharomyces cerevisiae BY4741
-
L-methionine + dithiothreitol disulfide + H2O
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae BY4741
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Talon-metal affinity column chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dabsyl-L-methionine-(S)-S-sulfoxide + dithiothreitol
-
Saccharomyces cerevisiae dabsyl-L-methionine + dithiothreitol disulfide + H2O
-
?
dabsyl-L-methionine-(S)-S-sulfoxide + dithiothreitol
-
Saccharomyces cerevisiae BY4741 dabsyl-L-methionine + dithiothreitol disulfide + H2O
-
?
L-methionine (S)-sulfoxide + 2 dithiothreitol
-
Saccharomyces cerevisiae L-methionine + dithiothreitol disulfide + H2O
-
?
L-methionine (S)-sulfoxide + 2 dithiothreitol
-
Saccharomyces cerevisiae BY4741 L-methionine + dithiothreitol disulfide + H2O
-
?

Synonyms

Synonyms Comment Organism
methionine sulfoxide reductase A
-
Saccharomyces cerevisiae
MsrA
-
Saccharomyces cerevisiae