Cloned (Comment) | Organism |
---|---|
- |
Alkaliphilus oremlandii |
Protein Variants | Comment | Organism |
---|---|---|
C15S | monothiol mutant | Alkaliphilus oremlandii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
glutaredoxin 2 | wild-type, pH 7.5, 37°C | Alkaliphilus oremlandii | |
0.27 | - |
glutaredoxin 2 | mutant C15S, pH 7.5, 37°C | Alkaliphilus oremlandii | |
0.84 | - |
dithiothreitol | wild-type, pH 7.5, 37°C | Alkaliphilus oremlandii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alkaliphilus oremlandii | A8MI53 | - |
- |
Alkaliphilus oremlandii OhILAs | A8MI53 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-methionine (S)-sulfoxide + 2 dithiothreitol | - |
Alkaliphilus oremlandii | L-methionine + dithiothreitol disulfide + H2O | - |
? | |
L-methionine (S)-sulfoxide + 2 dithiothreitol | - |
Alkaliphilus oremlandii OhILAs | L-methionine + dithiothreitol disulfide + H2O | - |
? | |
L-methionine (S)-sulfoxide + glutaredoxin 2 | - |
Alkaliphilus oremlandii | L-methionine + glutaredoxin 2 disulfide + H2O | - |
? | |
L-methionine (S)-sulfoxide + glutaredoxin 2 | - |
Alkaliphilus oremlandii OhILAs | L-methionine + glutaredoxin 2 disulfide + H2O | - |
? | |
additional information | dunring the regeneration mechanism of MsrA by glutaredoxin, the catalytic Cys16 attacks the sulfoxide moiety of the substrate to form a sulfenic acid intermediate with the concomitant release of the product methionine. The catalytic Cys16 sulfenic acid is then attacked by glutaredoxin, leading to the formation of a complex through a mixed disulfide bond, which is reduced by glutathione, leading to MsrA regeneration | Alkaliphilus oremlandii | ? | - |
? | |
additional information | dunring the regeneration mechanism of MsrA by glutaredoxin, the catalytic Cys16 attacks the sulfoxide moiety of the substrate to form a sulfenic acid intermediate with the concomitant release of the product methionine. The catalytic Cys16 sulfenic acid is then attacked by glutaredoxin, leading to the formation of a complex through a mixed disulfide bond, which is reduced by glutathione, leading to MsrA regeneration | Alkaliphilus oremlandii OhILAs | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.5 | - |
glutaredoxin 2 | wild-type, pH 7.5, 37°C | Alkaliphilus oremlandii | |
4.6 | - |
glutaredoxin 2 | mutant C15S, pH 7.5, 37°C | Alkaliphilus oremlandii | |
10 | - |
dithiothreitol | wild-type, pH 7.5, 37°C | Alkaliphilus oremlandii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12 | - |
dithiothreitol | wild-type, pH 7.5, 37°C | Alkaliphilus oremlandii | |
17 | - |
glutaredoxin 2 | mutant C15S, pH 7.5, 37°C | Alkaliphilus oremlandii | |
18 | - |
glutaredoxin 2 | wild-type, pH 7.5, 37°C | Alkaliphilus oremlandii |