Cloned (Comment) | Organism |
---|---|
overexpression of wild-type and mutant enzymes in Escherichia coli | Neisseria meningitidis |
Protein Variants | Comment | Organism |
---|---|---|
C439S | site-directed mutagenesis, MsrA domain of PILB, mutant is inactive with thioredoxin, but about 10fold more active than the wild-type enzyme MsrA domain | Neisseria meningitidis |
C494S | site-directed mutagenesis, MsrA domain of PILB, inactive mutant | Neisseria meningitidis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Neisseria meningitidis | |
0.034 | - |
thioredoxin | MsrB activity of PILB, pH 8.0, 25°C | Neisseria meningitidis | |
56 | - |
L-methionine (R,S)-sulfoxide | MsrB activity of PILB, pH 8.0, 25°C | Neisseria meningitidis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | content of free cysteinyl residues in wild-type and mutant enzymes, MsrA and MsrB domains, overview | Neisseria meningitidis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
16374 | - |
x * 16374, about, recombinant wild-type MsrB domain, mass spectrometry | Neisseria meningitidis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neisseria meningitidis | - |
PilB enzyme has 2 catalytic domains showing MsrA, methionine S-oxide reductase (S-form oxidizing), and MsrB, methionine S-oxide reductase (R-form oxidizing), activity, respectively | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-methionine (R)-sulfoxide + thioredoxin = L-methionine + thioredoxin disulfide + H2O | reaction mechanism, Cys494 and Cys439 are involved | Neisseria meningitidis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3 | - |
recombinant wild-type MsrB domain, cosubstrate dithiothreitol | Neisseria meningitidis |
4.2 | - |
recombinant wild-type MsrA/MsrB, cosubstrate dithiothreitol | Neisseria meningitidis |
12 | - |
recombinant wild-type MsrB domain, cosubstrate thioredoxin | Neisseria meningitidis |
170 | - |
recombinant wild-type MsrA/MsrB, cosubstrate thioredoxin | Neisseria meningitidis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-methionine (R)-sulfoxide + thioredoxin | MsrB activity of the tandem domains of PilB, the MsrB domain alone does not utilize the S-isomer | Neisseria meningitidis | L-methionine + thioredoxin disulfide + H2O | - |
? | |
additional information | substrate specificity of MsrB activity, diverse substrates, overview | Neisseria meningitidis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 16374, about, recombinant wild-type MsrB domain, mass spectrometry | Neisseria meningitidis |
More | MsrB activity is located on the C-terminal domain of PILB, the fused domains are folded entities | Neisseria meningitidis |
Synonyms | Comment | Organism |
---|---|---|
methionine sulfoxide reductase | - |
Neisseria meningitidis |
MsrB | - |
Neisseria meningitidis |
PilB | - |
Neisseria meningitidis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.8 | - |
L-methionine (R,S)-sulfoxide | MsrB activity of PILB, pH 8.0, 25°C | Neisseria meningitidis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | - |
Neisseria meningitidis | |
thioredoxin | - |
Neisseria meningitidis |