Cloned (Comment) | Organism |
---|---|
overexpression of the MsrB domain in Escherichia coli, strain B834(DE3) produces a selenomethionine-substituted MsrB | Neisseria meningitidis |
Crystallization (Comment) | Organism |
---|---|
selenomethionine-substituted peptide methionine sulfoxide reductase B domain, hanging drop vapour diffusion method in multiwell tissue-culture plates, 0.004 ml protein solution containing 75 mg/ml protein in 50 mM Tris-HCl, pH 8.0, mixed with 0.004 ml precipitant solution at 20°C, 3 days, X-ray diffraction structure determination and analysis at 1.8 A resolution | Neisseria meningitidis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neisseria meningitidis | - |
enzyme PILB, a peptide methionine sulfoxide reductase with 2 subdomains MsrA and MsrB with opposite stereospecificity, MsrA activity belongs to EC 1.8.4.B2 | - |
Purification (Comment) | Organism |
---|---|
recombinant MsrB domain from Escherichia coli | Neisseria meningitidis |
Subunits | Comment | Organism |
---|---|---|
? | x * 16372.0, recombinant MsrB, mass spectrometry, x * 16467.2, recombinant selenomethionine-MsrB, mass spectrometry | Neisseria meningitidis |
Synonyms | Comment | Organism |
---|---|---|
MSR | - |
Neisseria meningitidis |
peptide methionine sulfoxide reductase | - |
Neisseria meningitidis |
PilB | - |
Neisseria meningitidis |