Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli | Neisseria meningitidis |
Protein Variants | Comment | Organism |
---|---|---|
C63S | site-directed mutagenesis, the mutant accumulates the sulfenic acid intermediate, while the wild-type accumulates the disulfide intermediate | Neisseria meningitidis |
W65F | site-directed mutagenesis, structural change of substrate binding and active site structure compared to the wild-type enzyme | Neisseria meningitidis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | stopped flow kinetics, steady-state kinetics | Neisseria meningitidis | |
0.026 | - |
thioredoxin | pH 5.5, 25°C | Neisseria meningitidis | |
0.14 | - |
acetyl-L-methionine (R)-sulfoxide N-methyl ester | pH 5.5, 25°C | Neisseria meningitidis | |
2.2 | - |
acetyl-L-methionine (R)-sulfoxide N-methyl ester | pH 8.0, 25°C | Neisseria meningitidis | |
7 | - |
thioredoxin | pH 8.0, 25°C | Neisseria meningitidis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neisseria meningitidis | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-methionine (R)-sulfoxide + thioredoxin = L-methionine + thioredoxin disulfide + H2O | three-step catalytic mechanism, influence of pH on reaction mechanism, overview | Neisseria meningitidis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-L-methionine (R)-sulfoxide methyl ester + thioredoxin | the affinity of MsrB to acetyl-L-methionine (R)-sulfoxide methyl ester is higher than to L-methionine (R)-sulfoxide | Neisseria meningitidis | L-methionine methyl ester + thioredoxin disulfide + H2O | - |
? | |
acetyl-L-methionine (R)-sulfoxide N-methyl ester + thioredoxin | - |
Neisseria meningitidis | L-methionine methyl ester + thioredoxin disulfide + H2O | - |
r | |
L-methionine (R)-sulfoxide + thioredoxin | MsrB specifically reduces the R-form of methionine sulfoxide | Neisseria meningitidis | L-methionine + thioredoxin disulfide + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Neisseria meningitidis |
Synonyms | Comment | Organism |
---|---|---|
methionine sulfoxide reductase B | - |
Neisseria meningitidis |
MsrB | - |
Neisseria meningitidis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Neisseria meningitidis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thioredoxin | dependent on | Neisseria meningitidis |