Application | Comment | Organism |
---|---|---|
synthesis | enzyme can be useful in the development and action of anti-cancer and anti-inflammation drugs | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
gene msr or pilB, DNA sequence determination and analysis | Neisseria gonorrhoeae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a msrA/msrB double mutant for detection of additional enzyme form activities | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | Sel-X, a MsrB enzyme form variant | Homo sapiens | 5829 | - |
cytosol | Sel-X, a MsrB enzyme form variant | Saccharomyces cerevisiae | 5829 | - |
cytosol | Sel-X, a MsrB enzyme form variant, sulindac reducing activity in calf | Bos taurus | 5829 | - |
cytosol | Sel-X, a MsrB enzyme variant | Mus musculus | 5829 | - |
membrane | enzyme form Mem-R,S-Msr | Escherichia coli | 16020 | - |
microsome | calf, sulindac reducing activity | Bos taurus | - |
- |
mitochondrion | Sel-X, a MsrB enzyme form variant | Homo sapiens | 5739 | - |
mitochondrion | Sel-X, a MsrB enzyme form variant | Saccharomyces cerevisiae | 5739 | - |
mitochondrion | Sel-X, a MsrB enzyme form variant, sulindac reducing activity in calf | Bos taurus | 5739 | - |
mitochondrion | Sel-X, a MsrB enzyme variant | Mus musculus | 5739 | - |
additional information | subcellular sulindac reducing activity distribution in calf liver | Bos taurus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
selenium | selenoprotein | Saccharomyces cerevisiae | |
selenium | selenoprotein | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
x * 57000, MsrA/B | Neisseria gonorrhoeae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-methionine (R)-sulfoxide + thioredoxin | Bos taurus | enzyme form MsrB is specific for the R-form, enzyme form variants with specificities for either free or protein-bound methionine | L-methionine + thioredoxin disulfide + H2O | - |
? | |
L-methionine (R)-sulfoxide + thioredoxin | Escherichia coli | membrane-bound enzyme form Mem-R,S-Msr, enzyme form MsrB is specific for the R-form, MsrB enzyme form variants with specificities for either free or protein-bound methionine, Mem-R,S-Msr also posesses MsrA activity utilizing L-methionine (S)-sulfoxide as substrate | L-methionine + thioredoxin disulfide + H2O | - |
? | |
L-methionine (R)-sulfoxide + thioredoxin | Mus musculus | MsrB is specific for the R-form, enzyme variants with specificities for either free or protein-bound methionine | L-methionine + thioredoxin disulfide + H2O | - |
? | |
L-methionine (R)-sulfoxide + thioredoxin | Homo sapiens | MsrB is specific for the R-form, enzyme variants with specificities for either free or protein-bound methionine | L-methionine + thioredoxin disulfide + H2O | - |
? | |
L-methionine (R)-sulfoxide + thioredoxin | Saccharomyces cerevisiae | MsrB is specific for the R-form, enzyme variants with specificities for either free or protein-bound methionine | L-methionine + thioredoxin disulfide + H2O | - |
? | |
L-methionine (R,S)-sulfoxide + thioredoxin | Haemophilus influenzae | the enzyme protects cells against oxidative damage and plays a role in age-related misfunctions | L-methionine + thioredoxin disulfide | - |
? | |
L-methionine (R,S)-sulfoxide + thioredoxin | Neisseria gonorrhoeae | the enzyme protects cells against oxidative damage and plays a role in age-related misfunctions | L-methionine + thioredoxin disulfide | - |
? | |
L-methionine (R,S)-sulfoxide + thioredoxin | Neisseria meningitidis | the enzyme protects cells against oxidative damage and plays a role in age-related misfunctions | L-methionine + thioredoxin disulfide | - |
? | |
L-methionine (R,S)-sulfoxide + thioredoxin | Streptococcus pneumoniae | the enzyme protects cells against oxidative damage and plays a role in age-related misfunctions | L-methionine + thioredoxin disulfide | - |
? | |
L-methionine (R,S)-sulfoxide + thioredoxin | Helicobacter pylori | the enzyme protects cells against oxidative damage and plays a role in age-related misfunctions | L-methionine + thioredoxin disulfide | - |
? | |
L-methionine (R,S)-sulfoxide + thioredoxin | Streptococcus gordonii | the enzyme protects cells against oxidative damage and plays a role in age-related misfunctions | L-methionine + thioredoxin disulfide | - |
? | |
additional information | Mus musculus | the enzyme protect cells against oxidative damage and plays a role in age-related diseases | ? | - |
? | |
additional information | Escherichia coli | the enzyme protects cells against oxidative damage and plays a role in age-related misfunctions | ? | - |
? | |
additional information | Homo sapiens | the enzyme protects cells against oxidative damage and plays a role in age-related and neurological diseases, like Parkinsons or Alzheimers disease | ? | - |
? | |
additional information | Saccharomyces cerevisiae | the enzyme protects cells against oxidative damage and plays a role in age-related diseases | ? | - |
? | |
additional information | Bos taurus | the enzyme protects cells against oxidative damage and plays a role in age-related diseases | ? | - |
? | |
sulindac + thioredoxin | Bos taurus | activation of a methionine sulfoxide-containing prodrug, activity with membrane-bound enzyme form Mem-R,S-Msr | sulindac sulfide + thioredoxin disulfide | activated drug which inhibits cyclooxygenase 1 and 2 and exhibiting anti-inflammatory activity | ? | |
sulindac + thioredoxin | Escherichia coli | activation of a methionine sulfoxide-containing prodrug, activity with membrane-bound enzyme form Mem-R,S-Msr and MsrA | sulindac sulfide + thioredoxin disulfide | activated drug which inhibits cyclooxygenase 1 and 2 and exhibiting anti-inflammatory activity | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
MsrB variants one of which is also called Sel-X | - |
Escherichia coli | - |
enzyme forms MsrB and Mem-R,S-Msr | - |
Haemophilus influenzae | - |
- |
- |
Haemophilus influenzae | - |
gene msr encodes an enzyme showing both MsrA, methionine S-oxide reductase (S-form oxidizing), and MsrB, methionine S-oxide reductase (R-form oxidizing), activity | - |
Helicobacter pylori | - |
- |
- |
Helicobacter pylori | - |
gene msr encodes an enzyme showing both MsrA, methionine S-oxide reductase (S-form oxidizing), and MsrB, methionine S-oxide reductase (R-form oxidizing), activity | - |
Homo sapiens | - |
MsrB variants one of which is also called Sel-X, encoded by distinct genes, MsrB is a selenoprotein | - |
Mus musculus | - |
MsrB is a selenoprotein | - |
Neisseria gonorrhoeae | - |
- |
- |
Neisseria gonorrhoeae | - |
gene msr encodes an enzyme showing both MsrA, methionine S-oxide reductase (S-form oxidizing), and MsrB, methionine S-oxide reductase (R-form oxidizing), activity | - |
Neisseria meningitidis | - |
- |
- |
Neisseria meningitidis | - |
gene msr encodes an enzyme showing both MsrA, EC 1.8.4.B2, and MsrB activity | - |
Saccharomyces cerevisiae | - |
MsrB variants one of which is also called Sel-X | - |
Streptococcus gordonii | - |
gene msr encodes an enzyme showing both MsrA, methionine S-oxide reductase (S-form oxidizing), and MsrB, methionine S-oxide reductase (R-form oxidizing), activity | - |
Streptococcus gordonii | Q9LAM9 | - |
- |
Streptococcus pneumoniae | - |
- |
- |
Streptococcus pneumoniae | - |
gene msr encodes an enzyme showing both MsrA, methionine S-oxide reductase (S-form oxidizing), and MsrB, methionine S-oxide reductase (R-form oxidizing), activity | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-methionine (R)-sulfoxide + thioredoxin = L-methionine + thioredoxin disulfide + H2O | catalytic mechanism involving the formation of a sulfenic acid intermediate | Escherichia coli | |
L-methionine (R)-sulfoxide + thioredoxin = L-methionine + thioredoxin disulfide + H2O | catalytic mechanism involving the formation of a sulfenic acid intermediate | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | calf, sulindac reducing activity | Bos taurus | - |
kidney | calf, sulindac reducing activity | Bos taurus | - |
liver | calf, sulindac reducing activity | Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
subcellular sulindac reducing activity in calf liver | Bos taurus |
0.00009 | - |
enzyme form Mem-R,S-Msr, substrate sulindac | Escherichia coli |
0.0048 | - |
wild-type strain, substrate L-methionine (R)-sulfoxide | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-methionine (R)-sulfoxide + thioredoxin | enzyme form MsrB is specific for the R-form, enzyme form variants with specificities for either free or protein-bound methionine | Bos taurus | L-methionine + thioredoxin disulfide + H2O | - |
? | |
L-methionine (R)-sulfoxide + thioredoxin | membrane-bound enzyme form Mem-R,S-Msr, enzyme form MsrB is specific for the R-form, MsrB enzyme form variants with specificities for either free or protein-bound methionine, Mem-R,S-Msr also posesses MsrA activity utilizing L-methionine (S)-sulfoxide as substrate | Escherichia coli | L-methionine + thioredoxin disulfide + H2O | - |
? | |
L-methionine (R)-sulfoxide + thioredoxin | MsrB is specific for the R-form, enzyme variants with specificities for either free or protein-bound methionine | Mus musculus | L-methionine + thioredoxin disulfide + H2O | - |
? | |
L-methionine (R)-sulfoxide + thioredoxin | MsrB is specific for the R-form, enzyme variants with specificities for either free or protein-bound methionine | Homo sapiens | L-methionine + thioredoxin disulfide + H2O | - |
? | |
L-methionine (R)-sulfoxide + thioredoxin | MsrB is specific for the R-form, enzyme variants with specificities for either free or protein-bound methionine | Saccharomyces cerevisiae | L-methionine + thioredoxin disulfide + H2O | - |
? | |
L-methionine (R)-sulfoxide + thioredoxin | MsrB is specific for the R-form, enzyme variants with specificities for either free or protein-bound methionine | Bos taurus | L-methionine + thioredoxin disulfide + H2O | - |
? | |
L-methionine (R)-sulfoxide + thioredoxin | membrane-bound enzyme form Mem-R,S-Msr | Escherichia coli | L-methionine + thioredoxin disulfide + H2O | - |
? | |
L-methionine (R,S)-sulfoxide + thioredoxin | the enzyme protects cells against oxidative damage and plays a role in age-related misfunctions | Haemophilus influenzae | L-methionine + thioredoxin disulfide | - |
? | |
L-methionine (R,S)-sulfoxide + thioredoxin | the enzyme protects cells against oxidative damage and plays a role in age-related misfunctions | Neisseria gonorrhoeae | L-methionine + thioredoxin disulfide | - |
? | |
L-methionine (R,S)-sulfoxide + thioredoxin | the enzyme protects cells against oxidative damage and plays a role in age-related misfunctions | Neisseria meningitidis | L-methionine + thioredoxin disulfide | - |
? | |
L-methionine (R,S)-sulfoxide + thioredoxin | the enzyme protects cells against oxidative damage and plays a role in age-related misfunctions | Streptococcus pneumoniae | L-methionine + thioredoxin disulfide | - |
? | |
L-methionine (R,S)-sulfoxide + thioredoxin | the enzyme protects cells against oxidative damage and plays a role in age-related misfunctions | Helicobacter pylori | L-methionine + thioredoxin disulfide | - |
? | |
L-methionine (R,S)-sulfoxide + thioredoxin | the enzyme protects cells against oxidative damage and plays a role in age-related misfunctions | Streptococcus gordonii | L-methionine + thioredoxin disulfide | - |
? | |
L-methionine (R,S)-sulfoxide + thioredoxin | enzyme MsrA/B shows both MsrA and MsrB activity, free and protein-bound methionine | Haemophilus influenzae | L-methionine + thioredoxin disulfide | - |
? | |
L-methionine (R,S)-sulfoxide + thioredoxin | enzyme MsrA/B shows both MsrA and MsrB activity, free and protein-bound methionine | Neisseria gonorrhoeae | L-methionine + thioredoxin disulfide | - |
? | |
L-methionine (R,S)-sulfoxide + thioredoxin | enzyme MsrA/B shows both MsrA and MsrB activity, free and protein-bound methionine | Neisseria meningitidis | L-methionine + thioredoxin disulfide | - |
? | |
L-methionine (R,S)-sulfoxide + thioredoxin | enzyme MsrA/B shows both MsrA and MsrB activity, free and protein-bound methionine | Streptococcus pneumoniae | L-methionine + thioredoxin disulfide | - |
? | |
L-methionine (R,S)-sulfoxide + thioredoxin | enzyme MsrA/B shows both MsrA and MsrB activity, free and protein-bound methionine | Helicobacter pylori | L-methionine + thioredoxin disulfide | - |
? | |
L-methionine (R,S)-sulfoxide + thioredoxin | enzyme MsrA/B shows both MsrA and MsrB activity, free and protein-bound methionine | Streptococcus gordonii | L-methionine + thioredoxin disulfide | - |
? | |
L-methionine sulfoxide enkephalin + thioredoxin | membrane-bound enzyme form Mem-R,S-Msr | Escherichia coli | L-methionine enkephalin | - |
? | |
additional information | the enzyme protect cells against oxidative damage and plays a role in age-related diseases | Mus musculus | ? | - |
? | |
additional information | the enzyme protects cells against oxidative damage and plays a role in age-related misfunctions | Escherichia coli | ? | - |
? | |
additional information | the enzyme protects cells against oxidative damage and plays a role in age-related and neurological diseases, like Parkinsons or Alzheimers disease | Homo sapiens | ? | - |
? | |
additional information | the enzyme protects cells against oxidative damage and plays a role in age-related diseases | Saccharomyces cerevisiae | ? | - |
? | |
additional information | the enzyme protects cells against oxidative damage and plays a role in age-related diseases | Bos taurus | ? | - |
? | |
additional information | enzyme reduces oxidized methionine residues of the alpha-1-proteinase inhibitor, calmodulin, and thrombomodulin, which become reversibly inactivated upon oxidation | Homo sapiens | ? | - |
? | |
additional information | substrate specificity and activity of MsrB/PilB in comparison to MsrA, overview | Neisseria gonorrhoeae | ? | - |
? | |
additional information | substrate specificity of the different enzyme forms, overview, enzyme reduces oxidized methionine residues of the ribosomal protein L12, which becomes reversibly inactivated and forms monomers instead of dimers upon oxidation, Mem-R,S-Msr also posesses MsrA activity utilizing L-methionine (S)-sulfoxide as substrate | Escherichia coli | ? | - |
? | |
N-acetyl-L-methionine (R,S)-sulfoxide + thioredoxin | enzyme MsrA/B shows both MsrA and MsrB activity, free and protein-bound methionine | Neisseria gonorrhoeae | N-acetyl-L-methionine + thioredoxin disulfide | - |
? | |
N-acetyl-L-methionine (R,S)-sulfoxide + thioredoxin | membrane-bound enzyme form Mem-R,S-Msr | Escherichia coli | N-acetyl-L-methionine + thioredoxin disulfide | - |
? | |
sulindac + thioredoxin | activation of a methionine sulfoxide-containing prodrug, activity with membrane-bound enzyme form Mem-R,S-Msr | Bos taurus | sulindac sulfide + thioredoxin disulfide | activated drug which inhibits cyclooxygenase 1 and 2 and exhibiting anti-inflammatory activity | ? | |
sulindac + thioredoxin | activation of a methionine sulfoxide-containing prodrug, activity with membrane-bound enzyme form Mem-R,S-Msr and MsrA | Escherichia coli | sulindac sulfide + thioredoxin disulfide | activated drug which inhibits cyclooxygenase 1 and 2 and exhibiting anti-inflammatory activity | ? | |
sulindac + thioredoxin | activity with membrane-bound enzyme form Mem-R,S-Msr | Bos taurus | sulindac sulfide + thioredoxin disulfide | - |
? | |
sulindac + thioredoxin | activity with membrane-bound enzyme form Mem-R,S-Msr and MsrA | Escherichia coli | sulindac sulfide + thioredoxin disulfide | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 57000, MsrA/B | Neisseria gonorrhoeae |
Synonyms | Comment | Organism |
---|---|---|
methionine sulfoxide reductase | - |
Haemophilus influenzae |
methionine sulfoxide reductase | - |
Mus musculus |
methionine sulfoxide reductase | - |
Escherichia coli |
methionine sulfoxide reductase | - |
Homo sapiens |
methionine sulfoxide reductase | - |
Saccharomyces cerevisiae |
methionine sulfoxide reductase | - |
Bos taurus |
methionine sulfoxide reductase | - |
Neisseria gonorrhoeae |
methionine sulfoxide reductase | - |
Neisseria meningitidis |
methionine sulfoxide reductase | - |
Streptococcus pneumoniae |
methionine sulfoxide reductase | - |
Helicobacter pylori |
methionine sulfoxide reductase | - |
Streptococcus gordonii |
MSR | - |
Haemophilus influenzae |
MSR | - |
Escherichia coli |
MSR | - |
Neisseria meningitidis |
MSR | - |
Streptococcus pneumoniae |
MSR | - |
Helicobacter pylori |
MSR | - |
Streptococcus gordonii |
MsrA/B | - |
Neisseria gonorrhoeae |
MsrA/B | bifunctional enzyme: EC 1.8.4.11/EC 1.8.4.12 | Haemophilus influenzae |
MsrA/B | bifunctional enzyme: EC 1.8.4.11/EC 1.8.4.12 | Neisseria gonorrhoeae |
MsrA/B | bifunctional enzyme: EC 1.8.4.11/EC 1.8.4.12 | Neisseria meningitidis |
MsrA/B | bifunctional enzyme: EC 1.8.4.11/EC 1.8.4.12 | Streptococcus pneumoniae |
MsrA/B | bifunctional enzyme: EC 1.8.4.11/EC 1.8.4.12 | Helicobacter pylori |
MsrA/B | bifunctional enzyme: EC 1.8.4.11/EC 1.8.4.12 | Streptococcus gordonii |
MsrB | - |
Mus musculus |
MsrB | - |
Escherichia coli |
MsrB | - |
Homo sapiens |
MsrB | - |
Saccharomyces cerevisiae |
MsrB | - |
Bos taurus |
PilB | - |
Neisseria gonorrhoeae |
Sel-X | - |
Mus musculus |
Sel-X | - |
Homo sapiens |
Sel-X | - |
Saccharomyces cerevisiae |
Sel-X | - |
Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with DTT as cofactor by membrane-bound enzyme form Mem-R,S-Msr | Escherichia coli | |
NADPH | membrane-bound enzyme form Mem-R,S-Msr | Escherichia coli | |
thioredoxin | - |
Haemophilus influenzae | |
thioredoxin | - |
Mus musculus | |
thioredoxin | - |
Homo sapiens | |
thioredoxin | - |
Saccharomyces cerevisiae | |
thioredoxin | - |
Bos taurus | |
thioredoxin | - |
Neisseria gonorrhoeae | |
thioredoxin | - |
Neisseria meningitidis | |
thioredoxin | - |
Streptococcus pneumoniae | |
thioredoxin | - |
Helicobacter pylori | |
thioredoxin | - |
Streptococcus gordonii | |
thioredoxin | preferred cofactor | Escherichia coli |