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Literature summary for 1.8.4.12 extracted from

  • Skaar, E.P.; Tobiason, D.M.; Quick, J.; Judd, R.C.; Weissbach, H.; Etienne, F.; Brot, N.; Seifert, H.S.
    The outer membrane localization of the Neisseria gonorrhoeae MsrA/B is involved in survival against reactive oxygen species (2002), Proc. Natl. Acad. Sci. USA, 99, 10108-10113.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.8.4.12

Cloned(Commentary)

Cloned (Comment) Organism
gene pilB, transposon insertion, truncated PilB enzyme forms of the enzyme lacking the MsrA domain from strain MS11, variant VD300, overexpression of the His-tagged PilB forms in Escherichia coli strain Xl-1 blue Neisseria gonorrhoeae

Protein Variants

Protein Variants Comment Organism
additional information construction of truncated PilB domain forms, which show decreased survival of the organism to reactive oxygen species, the mutations do not affect piliation, pilin production, or adherence, overview Neisseria gonorrhoeae

Localization

Localization Comment Organism GeneOntology No. Textmining
outer membrane
-
 Neisseria gonorrhoeae 19867
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Neisseria gonorrhoeae PilB affects the survival of the organism to reactive oxygen species, PilB is not involved in piliation, pilin production, or adherence ?
-
 ?
additional information Neisseria gonorrhoeae MS11 PilB affects the survival of the organism to reactive oxygen species, PilB is not involved in piliation, pilin production, or adherence ?
-
 ?

Organism

Organism UniProt Comment Textmining
Neisseria gonorrhoeae
-
 variant VD300, bifunctional enzyme MsrA/B, gene pilB
-
Neisseria gonorrhoeae MS11
-
 variant VD300, bifunctional enzyme MsrA/B, gene pilB
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged PilB forms in Escherichia coli strain Xl-1 blue by nickel affinity chromatography Neisseria gonorrhoeae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information PilB affects the survival of the organism to reactive oxygen species, PilB is not involved in piliation, pilin production, or adherence Neisseria gonorrhoeae ?
-
 ?
additional information the bifunctional enzyme catalyzes both reactions of MsrB or PilB, EC 1.8.4.12, and of MsrA or PilA, EC 1.8.4.11, the catalytic sites for the two different activities are localized separatly on the enzyme molecule, overview Neisseria gonorrhoeae ?
-
 ?
additional information PilB affects the survival of the organism to reactive oxygen species, PilB is not involved in piliation, pilin production, or adherence Neisseria gonorrhoeae MS11 ?
-
 ?
additional information the bifunctional enzyme catalyzes both reactions of MsrB or PilB, EC 1.8.4.12, and of MsrA or PilA, EC 1.8.4.11, the catalytic sites for the two different activities are localized separatly on the enzyme molecule, overview Neisseria gonorrhoeae MS11 ?
-
 ?
N-acetyl-L-methionine-(R)-S-oxide + thioredoxin
-
 Neisseria gonorrhoeae N-acetyl-L-methionine + thioredoxin disulfide + H2O
-
 ?
N-acetyl-L-methionine-(R)-S-oxide + thioredoxin
-
 Neisseria gonorrhoeae MS11 N-acetyl-L-methionine + thioredoxin disulfide + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 15000-35000, recombinant His-tagged PilB forms, SDS-PAGE Neisseria gonorrhoeae

Synonyms

Synonyms Comment Organism
MsrB
-
 Neisseria gonorrhoeae
PilB
-
 Neisseria gonorrhoeae

Cofactor

Cofactor Comment Organism Structure
thioredoxin
-
 Neisseria gonorrhoeae