Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Neisseria meningitidis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | reductase step kinetic parameters of the wild-type and mutated MsrB | Neisseria meningitidis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neisseria meningitidis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Neisseria meningitidis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
peptide-L-methionine + thioredoxin disulfide + H2O = peptide-L-methionine (S)-S-oxide + thioredoxin | formation of the MsrB substrate complex leads to an activation of the catalytic Cys-117 characterized by a decreased pKapp of about 2.7 pH units. The catalytic active MsrB form is the Cys117-/His103+ species with a pKapp of 6.6 and 8.3, respectively. His103 and to a lesser extent His100, Asn119, and Thr26 (via a water molecule) participate in the stabilization of the polarized form of the sulfoxide function and of the transition state. Trp65 is essential for the catalytic efficiency of the reductase step by optimizing the position of the substrate in the active site | Neisseria meningitidis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-L-methionine-(R)-S-oxide-NHMe + thioredoxin | - |
Neisseria meningitidis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
methionine sulfoxide reductase B | - |
Neisseria meningitidis |
MsrB | - |
Neisseria meningitidis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | reductase step kinetic parameters of the wild-type and mutated MsrB | Neisseria meningitidis |