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Literature summary for 1.8.4.12 extracted from

  • Le, D.T.; Tarrago, L.; Watanabe, Y.; Kaya, A.; Lee, B.C.; Tran, U.; Nishiyama, R.; Fomenko, D.E.; Gladyshev, V.N.; Tran, L.S.
    Diversity of plant methionine sulfoxide reductases B and evolution of a form specific for free methionine sulfoxide (2013), PLoS ONE, 8, e65637.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.8.4.12

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells and in yeast cytosol Glycine max

Protein Variants

Protein Variants Comment Organism
C121S the mutant of isoform MSRB2 is inactive Glycine max
C68S the mutant of isoform MSRB2 is inactive Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.049
-
 N-acetyl-L-methionine (R)-sulfoxide isoform MSRB2, pH and temperature not specified in the publication Glycine max
0.049
-
 N-acetyl-L-methionine (R)-sulfoxide isoform MSRB4, pH and temperature not specified in the publication Glycine max
1.451
-
 L-methionine (R)-sulfoxide isoform MSRB4, pH and temperature not specified in the publication Glycine max
2.093
-
 L-methionine (R)-sulfoxide isoform MSRB2, pH and temperature not specified in the publication Glycine max

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-methionine (R)-sulfoxide + thioredoxin Glycine max
-
 L-methionine + thioredoxin disulfide + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Glycine max
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dabsyl-L-methionine (R)-sulfoxide + dithiothreitol isoforms MSRB2 and MSRB4 also show enzyme activity toward protein-based L-methionine (R)-sulfoxide with either dithiothreitol or thioredoxin as reductants, whereas isoform MSRB1 is active only with dithiothreitol Glycine max dabsyl-L-methionine + dithiothreitol disulfide + H2O
-
 ?
L-methionine (R)-sulfoxide + dithiothreitol
-
 Glycine max L-methionine + dithiothreitol disulfide + H2O
-
 ?
L-methionine (R)-sulfoxide + thioredoxin
-
 Glycine max L-methionine + thioredoxin disulfide + H2O
-
 ?
N-acetyl-L-methionine (R)-sulfoxide + dithiothreitol
-
 Glycine max N-acetyl-L-methionine + dithiothreitol disulfide + H2O
-
 ?

Synonyms

Synonyms Comment Organism
methionine sulfoxide reductase B
-
 Glycine max
MsrB2 isoform Glycine max
MSRB4 isoform Glycine max

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.13
-
 L-methionine (R)-sulfoxide isoform MSRB4, pH and temperature not specified in the publication Glycine max
0.24
-
 N-acetyl-L-methionine (R)-sulfoxide isoform MSRB4, pH and temperature not specified in the publication Glycine max
2.04
-
 N-acetyl-L-methionine (R)-sulfoxide isoform MSRB2, pH and temperature not specified in the publication Glycine max
2.86
-
 L-methionine (R)-sulfoxide isoform MSRB2, pH and temperature not specified in the publication Glycine max

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.09
-
 L-methionine (R)-sulfoxide isoform MSRB4, pH and temperature not specified in the publication Glycine max
1.4
-
 L-methionine (R)-sulfoxide isoform MSRB2, pH and temperature not specified in the publication Glycine max
5
-
 N-acetyl-L-methionine (R)-sulfoxide isoform MSRB4, pH and temperature not specified in the publication Glycine max
42
-
 N-acetyl-L-methionine (R)-sulfoxide isoform MSRB2, pH and temperature not specified in the publication Glycine max