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Literature summary for 1.8.4.12 extracted from
- Characterization of a methionine sulfoxide reductase B from tomato (Solanum lycopersicum), and its protecting role in Saccharomyces cerevisiae (2013), Protein J., 32, 39-47.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 (DE3)cells and in Saccharomyces cerevisiae strain BY4741 | Solanum lycopersicum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C181S | inactive | Solanum lycopersicum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0091 | - |
dithiothreitol | recombinant enzyme, in 50 mM Tris-HCl, pH 8.0, at 37°C | Solanum lycopersicum |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Solanum lycopersicum | 9507 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 21200 | - |
x * 21200, calculated from amino acid sequence | Solanum lycopersicum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Solanum lycopersicum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni2+-affinity column chromatography | Solanum lycopersicum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| flower | high expression | Solanum lycopersicum | - |
| fruit | high expression in red mature fruits | Solanum lycopersicum | - |
| leaf | high expression | Solanum lycopersicum | - |
| root | low expression | Solanum lycopersicum | - |
| stem | low expression | Solanum lycopersicum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dabsyl-L-methionine (R)-sulfoxide + dithiothreitol | - |
Solanum lycopersicum | dabsyl-L-methionine + dithiothreitol disulfide + H2O | - |
? | |
| L-methionine (R)-sulfoxide + dithiothreitol | - |
Solanum lycopersicum | L-methionine + dithiothreitol disulfide + H2O | - |
? | |
| additional information | isoform MSRB1 exhibits no activity in thioredoxin dependent system | Solanum lycopersicum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 21200, calculated from amino acid sequence | Solanum lycopersicum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| methionine sulfoxide reductase B | - |
Solanum lycopersicum |
| MsrB | - |
Solanum lycopersicum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.09 | - |
dithiothreitol | recombinant enzyme, in 50 mM Tris-HCl, pH 8.0, at 37°C | Solanum lycopersicum | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Solanum lycopersicum | calculated from amino acid sequence | - |
8.9 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the MSRB1 gene plays a critical role in protecting against oxidative stress | Solanum lycopersicum |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 10 | - |
dithiothreitol | recombinant enzyme, in 50 mM Tris-HCl, pH 8.0, at 37°C | Solanum lycopersicum | |
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Release 2023.1 (January 2023)
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