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Literature summary for 1.8.4.2 extracted from

  • Zaffagnini, M.; Michelet, L.; Massot, V.; Trost, P.; Lemaire, S.D.
    Biochemical characterization of glutaredoxins from Chlamydomonas reinhardtii reveals the unique properties of a chloroplastic CGFS-type glutaredoxin (2008), J. Biol. Chem., 283, 8868-8876 .
    View publication on PubMed
Search for more literature for 1.8.4.2

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00087
-
 glutathionylated glyceraldehyde-3-phosphate dehydrogenase S0.5 value, pH 7.9, temperature not specified in the publication Chlamydomonas reinhardtii
0.00454
-
 glutathionylated glyceraldehyde-3-phosphate dehydrogenase S0.5 value, pH 7.9, temperature not specified in the publication Chlamydomonas reinhardtii
2.65
-
 2-mercaptoethanol glutathione disulfide pH 7.9, temperature not specified in the publication Chlamydomonas reinhardtii

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Chlamydomonas reinhardtii 9507
-
cytosol
-
 Chlamydomonas reinhardtii 5829
-

Organism

Organism UniProt Comment Textmining
Chlamydomonas reinhardtii A8JH05 isoform GRX3, CGFS type enzyme
-
Chlamydomonas reinhardtii A8JHA9 isoform GRX1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 glutathione + insulin disulfide
-
 Chlamydomonas reinhardtii glutathione disulfide + insulin
-
 ?
2-mercaptoethanol glutathione disulfide + glutathione
-
 Chlamydomonas reinhardtii 2-mercaptoethanol disulfide + glutathione disulfide
-
 ?
dithiothreitol + insulin disulfide
-
 Chlamydomonas reinhardtii dithiothreitol disulfide + insulin
-
 ?
glutathionylated glyceraldehyde-3-phosphate dehydrogenase + glutathione substrate is glutathionylated A4-GAPDH. A4-GAPDH activity is reversibly inhibited by glutathionylation Chlamydomonas reinhardtii glutathione disulfide + glyceraldehyde-3-phosphate dehydrogenase
-
 ?
glutathionylated glyceraldehyde-3-phosphate dehydrogenase + reduced GRX3 substrate is glutathionylated A4-GAPDH. A4-GAPDH activity is reversibly inhibited by glutathionylation Chlamydomonas reinhardtii glutathione + glyceraldehyde-3-phosphate dehydrogenase + oxidized GRX3
-
 ?
additional information GRX1is a typical CPYC-type GRX, which is reduced by GSH and exhibits disulfide reductase, dehydroascorbate reductase, and deglutathionylation activities Chlamydomonas reinhardtii ?
-
-
additional information no substrate: insulin disulfide, 2-mercaptoethanol glutathione disulfide Chlamydomonas reinhardtii ?
-
-

Synonyms

Synonyms Comment Organism
GRX1
-
 Chlamydomonas reinhardtii
GRX3
-
 Chlamydomonas reinhardtii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
161.6
-
 2-mercaptoethanol glutathione disulfide pH 7.9, temperature not specified in the publication Chlamydomonas reinhardtii

General Information

General Information Comment Organism
physiological function CGFS-type GRX is not reduced by GSH and has an atypically low redox potential (-323 mV at pH 7.9). GRX3 can be reduced in the light by photoreduced ferredoxin and ferredoxin-thioredoxin reductase Chlamydomonas reinhardtii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
61
-
 2-mercaptoethanol glutathione disulfide pH 7.9, temperature not specified in the publication Chlamydomonas reinhardtii