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Literature summary for 1.8.4.4 extracted from
- A thiol-disulfide transhydrogenase from yeast (1968), J. Biol. Chem., 243, 1942-1947.
General Stability
| General Stability | Organism |
|---|---|
| ammonium sulfate, 1 M, complete stabilization, 6 days at 20°C | Saccharomyces cerevisiae |
| cystine, alone: no stabilization, in addition to glutathione: increases stabilizing effect | Saccharomyces cerevisiae |
| EDTA stabilizes | Saccharomyces cerevisiae |
| ethylene glycol, 10%, stabilizes | Saccharomyces cerevisiae |
| glutathione stabilizes, stabilizing effect increased by cystine | Saccharomyces cerevisiae |
| glycerol, no stabilization | Saccharomyces cerevisiae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell associated | - |
Saccharomyces cerevisiae | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 150000 | - |
gel filtration | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cystine + GSH | Saccharomyces cerevisiae | - |
cysteine + GSSG | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 61.6 | - |
beta-hydroxyethyldisulfide | Saccharomyces cerevisiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, pH 5.8, 1 mM glutathione, several months | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-hydroxyethyl disulfide + GSH | - |
Saccharomyces cerevisiae | ? | - |
? | |
| cystine + GSH | - |
Saccharomyces cerevisiae | cysteine + GSSG | - |
? | |
| D-cystine + 2 GSH | - |
Saccharomyces cerevisiae | 2 D-cysteine + GSSG | - |
? | |
| diacetyl-L-cystine + GSH | - |
Saccharomyces cerevisiae | ? | - |
? | |
| homocystine + GSH | - |
Saccharomyces cerevisiae | ? | - |
? | |
| L-cystine + GSH | - |
Saccharomyces cerevisiae | L-cysteine + GSSG | - |
? | |
| L-cystine diamide + GSH | - |
Saccharomyces cerevisiae | ? | - |
? | |
| L-cystinyldiglycine + GSH | - |
Saccharomyces cerevisiae | L-Cys-Gly-Gly + GSSG | - |
? | |
| additional information | at low substrate concentration the reaction rate is greater with L-cystine than with any of the other substances | Saccharomyces cerevisiae | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 23 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.6 | - |
pH 7.8 chosen for the assay to achieve smaller nonenzymatic rate | Saccharomyces cerevisiae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8.5 | pH 6.0: 10% of activity maximum, pH 8.5: activity maximum | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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