Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | insensitive against 1 mM CuSO4 and 1 mM N-ethylmaleimide at 37°C for 30 min | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
1 * 40000, SDS-PAGE | Rattus norvegicus |
43000 | - |
gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
newborn and adults | - |
Purification (Comment) | Organism |
---|---|
ion-exchange, gel filtration | Rattus norvegicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
[xanthine dehydrogenase] + glutathione disulfide = [xanthine oxidase] + 2 glutathione | thioltransferase, GSH:insulin transhydrogenase and ricin-disulphide-bond reductase activity | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
heart | - |
Rattus norvegicus | - |
kidney | - |
Rattus norvegicus | - |
liver | highest amount of activity | Rattus norvegicus | - |
lung | - |
Rattus norvegicus | - |
skeletal muscle | - |
Rattus norvegicus | - |
skin | - |
Rattus norvegicus | - |
spleen | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
372 U/mg, other definition of units | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | other disulfide compounds are either inactive or far less active than oxidized glutathione, reduction of disulfide bonds in ricin, thioltransferase activity, GSH:insulin transhydrogenase activity | Rattus norvegicus | ? | - |
? | |
[xanthine dehydrogenase] + oxidized glutathione | conversion into oxidase by treatment with proteolytic enzymes, storage at -20°C/-25°C or preincubation with either thiol reagents or whole liver homogenate. Can act in the opposite direction in the reduction of the disulfide bond of insulin and ricin | Rattus norvegicus | [xanthine oxidase] + glutathione | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 40000, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.1 | - |
assay at | Rattus norvegicus |